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MetaCyc Polypeptide: predicted carbon-phosphorus lyase subunit
Inferred from experiment

Gene: phnK Accession Numbers: EG10720 (MetaCyc), b4097, ECK4090

Species: Escherichia coli K-12 substr. MG1655

PhnK is a component of a protein complex that may be a carbon-phosphorus lyase [Jochimsen11].

phnK is part of an operon that is phosphate starvation-inducible and required for use of phosphonate and phosphite as phosphorous sources [Metcalf91, Chen90]. PhnK appears to be required for carbon-phosphorous lyase activity [Metcalf93]. A phnK mutant accumulates presumed intermediates of the C-P lyase pathway of phosphonate degradation [HoveJensen10].

Locations: cytosol

Map Position: [4,316,029 <- 4,316,787]

Molecular Weight of Polypeptide: 27.831 kD (from nucleotide sequence), 29.0 kD (experimental) [Jochimsen11]

Unification Links: ASAP:ABE-0013424, CGSC:34532, DIP:DIP-10490N, EchoBASE:EB0714, EcoGene:EG10720, EcoliWiki:b4097, ModBase:P16678, OU-Microarray:b4097, PortEco:phnK, Pride:P16678, Protein Model Portal:P16678, RefSeq:YP_026282, RegulonDB:EG10720, SMR:P16678, String:511145.b4097, UniProt:P16678

Relationship Links: InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR012700, InterPro:IN-FAMILY:IPR013563, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, Pfam:IN-FAMILY:PF00005, Pfam:IN-FAMILY:PF08352, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

GO Terms:
Biological Process:
Inferred from experimentGO:0019700 - organic phosphonate catabolic process [Metcalf93]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005829 - cytosol [DiazMejia09]

MultiFun Terms: metabolismmetabolism of other compoundsphosphorous metabolism

Imported from EcoCyc 20-Dec-2011 by Caspi R, SRI International

Sequence Features

Feature Class Location Attached Group Citations Comment
Conserved-Region 6 -> 246  
Inferred by computational analysis[UniProt15]
UniProt: ABC transporter.
Nucleotide-Phosphate-Binding-Region 38 -> 45 ATP
Inferred by computational analysis[UniProt15]
UniProt: ATP.
Sequence-Conflict 47  
Inferred by curator[Burland95, UniProt15]
UniProt: (in Ref. 3; AAA96996).

10/20/97 Gene b4097 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10720; confirmed by SwissProt match.


Burland95: Burland V, Plunkett G, Sofia HJ, Daniels DL, Blattner FR (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23(12);2105-19. PMID: 7610040

Chen90: Chen CM, Ye QZ, Zhu ZM, Wanner BL, Walsh CT (1990). "Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." J Biol Chem 265(8);4461-71. PMID: 2155230

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

HoveJensen10: Hove-Jensen B, Rosenkrantz TJ, Zechel DL, Willemoes M (2010). "Accumulation of intermediates of the carbon-phosphorus lyase pathway for phosphonate degradation in phn mutants of Escherichia coli." J Bacteriol 192(1);370-4. PMID: 19854894

Jochimsen11: Jochimsen B, Lolle S, McSorley FR, Nabi M, Stougaard J, Zechel DL, Hove-Jensen B (2011). "Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathway." Proc Natl Acad Sci U S A 108(28);11393-8. PMID: 21705661

Metcalf91: Metcalf WW, Wanner BL (1991). "Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi." J Bacteriol 173(2);587-600. PMID: 1846145

Metcalf93: Metcalf WW, Wanner BL (1993). "Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA' elements." J Bacteriol 175(11);3430-42. PMID: 8388873

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yakovleva98: Yakovleva GM, Kim SK, Wanner BL (1998). "Phosphate-independent expression of the carbon-phosphorus lyase activity of Escherichia coli." Appl Microbiol Biotechnol 49(5);573-8. PMID: 9650256

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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