|Gene:||phnK||Accession Numbers: EG10720 (MetaCyc), b4097, ECK4090|
Species: Escherichia coli K-12 substr. MG1655
Component of: predicted carbon-phosphorous lyase complex (summary available)
PhnK is a component of a protein complex that may be a carbon-phosphorous lyase [Jochimsen11].
phnK is part of an operon that is phosphate starvation-inducible and required for use of phosphonate and phosphite as phosphorous sources [Metcalf91, Chen90a]. PhnK appears to be required for carbon-phosphorous lyase activity [Metcalf93]. A phnK mutant accumulates presumed intermediates of the C-P lyase pathway of phosphonate degradation [HoveJensen10].
|Map Position: [4,316,029 <- 4,316,787]|
Molecular Weight of Polypeptide: 27.831 kD (from nucleotide sequence), 29.0 kD (experimental) [Jochimsen11 ]
Unification Links: ASAP:ABE-0013424 , CGSC:34532 , DIP:DIP-10490N , EchoBASE:EB0714 , EcoGene:EG10720 , EcoliWiki:b4097 , ModBase:P16678 , OU-Microarray:b4097 , PortEco:phnK , Pride:P16678 , Protein Model Portal:P16678 , RefSeq:YP_026282 , RegulonDB:EG10720 , SMR:P16678 , String:511145.b4097 , UniProt:P16678
Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR012700 , InterPro:IN-FAMILY:IPR013563 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Pfam:IN-FAMILY:PF08352 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382
|Biological Process:||GO:0019700 - organic phosphonate catabolic process
GO:0006200 - ATP catabolic process [GOA et al., 2001]
GO:0006810 - transport [UniProt-GOA, 2011]
GO:0015833 - peptide transport [GOA et al., 2001]
|Molecular Function:||GO:0000166 - nucleotide binding
[UniProt-GOA, 2011, GOA et al., 2001]
GO:0005524 - ATP binding [UniProt-GOA, 2011, GOA et al., 2001]
GO:0016887 - ATPase activity [GOA et al., 2001]
|Cellular Component:||GO:0005829 - cytosol [Diaz-Mejia et al., 2009]|
|MultiFun Terms:||metabolism → metabolism of other compounds → phosphorous metabolism|
Subunit of: predicted carbon-phosphorous lyase complex
Species: Escherichia coli K-12 substr. MG1655
Subunit composition of
predicted carbon-phosphorous lyase complex = [PhnG]4[PhnH]2[PhnI]2[PhnK][PhnJ]2
PhnG subunit of methylphosphonate degradation complex = PhnG (summary available)
PhnH subunit of methylphosphonate degradation complex = PhnH (summary available)
PhnI subunit of methylphosphonate degradation complex = PhnI (summary available)
predicted carbon-phosphorous lyase subunit = PhnK (summary available)
carbon-phosphorous lyase = PhnJ (summary available)
The PhnGHIJK complex is predicted to perform a catalytic activity during utilization of phosphonates. However, using a variety of possible substrates and cofactors, no activity was found [Jochimsen11].
The utilization of phosphonates as the sole source of phosphate is "cryptic" in E. coli K-12 [Wanner90]. This is due to an 8 bp insertion in the phnE ORF that leads to a frameshift and premature termination of translation of PhnE. Spontanteous revertants have lost the 8 bp insertion [Makino91].
The genes encoding proteins of this complex are members of the 14-gene phnCDEFGHIJKLMNOP operon which is involved in phosphonate uptake and metabolism and is a member of the phosphate regulon [Metcalf91].
Molecular Weight: 260.0 kD (experimental) [Jochimsen11]
|Conserved-Region||6 -> 246|
|Nucleotide-Phosphate-Binding-Region||38 -> 45|
10/20/97 Gene b4097 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10720; confirmed by SwissProt match.
Burland95: Burland V, Plunkett G, Sofia HJ, Daniels DL, Blattner FR (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23(12);2105-19. PMID: 7610040
Chen90a: Chen CM, Ye QZ, Zhu ZM, Wanner BL, Walsh CT (1990). "Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." J Biol Chem 265(8);4461-71. PMID: 2155230
Diaz-Mejia et al., 2009: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
HoveJensen10: Hove-Jensen B, Rosenkrantz TJ, Zechel DL, Willemoes M (2010). "Accumulation of intermediates of the carbon-phosphorus lyase pathway for phosphonate degradation in phn mutants of Escherichia coli." J Bacteriol 192(1);370-4. PMID: 19854894
Jochimsen11: Jochimsen B, Lolle S, McSorley FR, Nabi M, Stougaard J, Zechel DL, Hove-Jensen B (2011). "Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathway." Proc Natl Acad Sci U S A 108(28);11393-8. PMID: 21705661
Makino91: Makino K, Kim SK, Shinagawa H, Amemura M, Nakata A (1991). "Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12." J Bacteriol 173(8);2665-72. PMID: 1840580
Metcalf91: Metcalf WW, Wanner BL (1991). "Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi." J Bacteriol 173(2);587-600. PMID: 1846145
Metcalf93: Metcalf WW, Wanner BL (1993). "Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA' elements." J Bacteriol 175(11);3430-42. PMID: 8388873
Yakovleva98: Yakovleva GM, Kim SK, Wanner BL (1998). "Phosphate-independent expression of the carbon-phosphorus lyase activity of Escherichia coli." Appl Microbiol Biotechnol 49(5);573-8. PMID: 9650256
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