MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Arabidopsis thaliana col : glycerol-3-phosphate dehydrogenase (NAD-dependent) Inferred from experiment : At2G41540
Clostridium acetobutylicum : glycerol-3-phosphate dehydrogenase : gpsA
Escherichia coli K-12 substr. MG1655 : glycerol-3-phosphate dehydrogenase, biosynthetic : gpsA
Homo sapiens : cytoplasmic glycerol-3-phosphate dehydrogenase (NAD+) Inferred from experiment : GPD1
Saccharomyces cerevisiae : glycerol 3-phosphate dehydrogenase Inferred from experiment : GPD1

In Pathway: glycerol-3-phosphate shuttle , 1,3-propanediol biosynthesis (engineered) , superpathway of phosphatidate biosynthesis (yeast)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Direct generic reaction:
sn-glycerol 3-phosphate + NAD(P)+ ← glycerone phosphate + NAD(P)H + H+ (

Enzyme Commission Primary Name: glycerol-3-phosphate dehydrogenase (NAD+)

Enzyme Commission Synonyms: α-glycerol phosphate dehydrogenase (NAD+), α-glycerophosphate dehydrogenase (NAD+), glycerol 1-phosphate dehydrogenase, glycerol phosphate dehydrogenase (NAD+), glycerophosphate dehydrogenase (NAD+), hydroglycerophosphate dehydrogenase, L-α-glycerol phosphate dehydrogenase, L-α-glycerophosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase (ambiguous), NAD+-α-glycerophosphate dehydrogenase, NAD+-dependent glycerol phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-L-glycerol-3-phosphate dehydrogenase, NAD+-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase, glycerol-3-phosphate dehydrogenase (NAD+), L-glycerol-3-phosphate dehydrogenase (ambiguous)

Standard Gibbs Free Energy (ΔrG in kcal/mol): 4.1518555 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Also acts on propane-1,2-diol phosphate and glycerone sulfate (but with a much lower affinity).

Citations: [Albertyn92, Koekemoer95, Warkentin73, OBrien72, Brosemer69]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reaction of [sn-glycerol 3-phosphate + NAD(P)+ ← glycerone phosphate + NAD(P)H + H+] (
i1: sn-glycerol 3-phosphate + NAD+ ↔ glycerone phosphate + NADH + H+ (

Unification Links: KEGG:R00842

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:O25614 , UniProt:RELATED-TO:O29390 , UniProt:RELATED-TO:P07735 , UniProt:RELATED-TO:P08507 , UniProt:RELATED-TO:P0A6S7 , UniProt:RELATED-TO:P13706 , UniProt:RELATED-TO:P13707 , UniProt:RELATED-TO:P21695 , UniProt:RELATED-TO:P34517 , UniProt:RELATED-TO:P41911 , UniProt:RELATED-TO:P43798 , UniProt:RELATED-TO:P46919 , UniProt:RELATED-TO:P90593 , UniProt:RELATED-TO:Q9JWH0 , UniProt:RELATED-TO:Q00055


Albertyn92: Albertyn J, van Tonder A, Prior BA (1992). "Purification and characterization of glycerol-3-phosphate dehydrogenase of Saccharomyces cerevisiae." FEBS Lett 308(2);130-2. PMID: 1499720

Brosemer69: Brosemer RW, Kuhn RW (1969). "Comparative structural properties of honeybee and rabbit alpha-glycerophosphate dehydrogenases." Biochemistry 8(5);2095-105. PMID: 4307630

Koekemoer95: Koekemoer TC, Litthauer D, Oelofsen W (1995). "Isolation and characterization of adipose tissue glycerol-3-phosphate dehydrogenase." Int J Biochem Cell Biol 27(6);625-32. PMID: 7671141

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

OBrien72: O'Brien SJ, MacIntyre RJ (1972). "The -glycerophosphate cycle in Drosophila melanogaster. I. Biochemical and developmental aspects." Biochem Genet 7(2);141-61. PMID: 4340553

Warkentin73: Warkentin DL, Fondy TP (1973). "Isolation and characterization of cytoplasmic L-glycerol-3-phosphate dehydrogenase from rabbit-renal-adipose tissue and its comparison with the skeletal-muscle enzyme." Eur J Biochem 36(1);97-109. PMID: 4200180

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Jul 30, 2015, biocyc12.