Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 1.1.99.28

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 1.1.99.28

Enzymes and Genes:
glucose-fructose oxidoreductase Inferred from experiment : gfo ( Zymomonas mobilis )

In Pathway: sorbitol biosynthesis II

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: glucose-fructose oxidoreductase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -5.630005 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
D-mannose, D-xylose, D-galactose, 2-deoxy-D-glucose and L-arabinose will function as aldose substrates, but with low affinities. The ketose substrate must be in the open-chain form. The apparent affinity for fructose is low, because little of the fructose substrate is in the open-chain form. Xylulose and glycerone (dihydroxyacetone) will replace fructose, but they are poor substrates. The enzyme from Zymomonas mobilis contains tightly bound NADP+.

Citations: [Zachariou86, Hardman88, Kanagasundaram92a]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R00874 , Rhea:20637

Relationship Links: BRENDA:EC:1.1.99.28 , ENZYME:EC:1.1.99.28 , IUBMB-ExplorEnz:EC:1.1.99.28


References

Hardman88: Hardman MJ, Scopes RK (1988). "The kinetics of glucose-fructose oxidoreductase from Zymomonas mobilis." Eur J Biochem 173(1);203-9. PMID: 3356190

Kanagasundaram92a: Kanagasundaram V, Scopes RK (1992). "Cloning, sequence analysis, and expression of the structural gene encoding glucose-fructose oxidoreductase from Zymomonas mobilis." J Bacteriol 174(5);1439-47. PMID: 1537789

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Zachariou86: Zachariou M, Scopes RK (1986). "Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production." J Bacteriol 167(3);863-9. PMID: 3745122


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.