MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Zymomonas mobilis: glucose-fructose oxidoreductaseInferred from experiment: gfo

In Pathway: sorbitol biosynthesis II

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: glucose-fructose oxidoreductase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -5.630005Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
D-mannose, D-xylose, D-galactose, 2-deoxy-D-glucose and L-arabinose will function as aldose substrates, but with low affinities. The ketose substrate must be in the open-chain form. The apparent affinity for fructose is low, because little of the fructose substrate is in the open-chain form. Xylulose and glycerone (dihydroxyacetone) will replace fructose, but they are poor substrates. The enzyme from Zymomonas mobilis contains tightly bound NADP+.

Citations: [Zachariou86, Hardman88, Kanagasundaram92]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R00874, Rhea:20637

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Hardman88: Hardman MJ, Scopes RK (1988). "The kinetics of glucose-fructose oxidoreductase from Zymomonas mobilis." Eur J Biochem 173(1);203-9. PMID: 3356190

Kanagasundaram92: Kanagasundaram V, Scopes RK (1992). "Cloning, sequence analysis, and expression of the structural gene encoding glucose-fructose oxidoreductase from Zymomonas mobilis." J Bacteriol 174(5);1439-47. PMID: 1537789

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Zachariou86: Zachariou M, Scopes RK (1986). "Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production." J Bacteriol 167(3);863-9. PMID: 3745122

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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