|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions → Protein-Modification Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Protein-Reactions → Protein-Modification Reactions|
EC Number: 22.214.171.124
Enzymes and Genes:
|Escherichia coli K-12 substr. MG1655 :||apo-citrate lyase phosphoribosyl-dephospho-CoA transferase
In Pathway: citrate lyase activation
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: citrate lyase holo-[acyl-carrier protein] synthase
Enzyme Commission Synonyms: 2'-(5''-phosphoribosyl)-3'-dephospho-CoA transferase, 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo-citrate lyase, CitX, holo-ACP synthase (ambiguous), 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo-citrate lyase adenylyltransferase, 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo-citrate lyase 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA transferase, 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo-citrate-lyase adenylyltransferase, holo-citrate lyase synthase (incorrect)
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -528.0613 [Latendresse13]
Enzyme Commission Summary:
The γ-subunit of EC 126.96.36.199, citrate (pro-3S)-lyase, serves as an acyl-carrier protein (ACP) and contains the prosthetic group 2'-(5''-triphospho-α-D-ribosyl)-3'-dephospho-CoA [Schneider00a, Schneider02a]. Synthesis and attachment of the prosthetic group requires the concerted action of this enzyme and EC 188.8.131.52, triphosphoribosyl-dephospho-CoA synthase [Schneider00a]. In the enzyme from E. coli, the prosthetic group is attached to serine-14 of the ACP via a phosphodiester bond.
Schneider00b: Schneider K, Dimroth P, Bott M (2000). "Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group." FEBS Lett 2000;483(2-3);165-8. PMID: 11042274
Schneider02a: Schneider K, Kastner CN, Meyer M, Wessel M, Dimroth P, Bott M (2002). "Identification of a gene cluster in Klebsiella pneumoniae which includes citX, a gene required for biosynthesis of the citrate lyase prosthetic group." J Bacteriol 2002;184(9);2439-46. PMID: 11948157
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493