Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Reaction: 3.1.1.76

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.1.1.76

Enzymes and Genes:
intracellular poly(3-hydroxyoctanoate) depolymerase Inferred from experiment : phaZ ( Pseudomonas putida KT2442 )
extracellular poly(3-hydroxyoctanoate) depolymerase Inferred from experiment : phaZ ( Pseudomonas fluorescens GK13 )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Undetermined; a substrate lacks a chemical formula

Enzyme Commission Primary Name: poly(3-hydroxyoctanoate) depolymerase

Enzyme Commission Synonyms: PHO depolymerase, poly(3HO) depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly(HA) depolymerase, poly(HAMCL) depolymerase, poly[(R)-3-hydroxyoctanoate] hydrolase

Enzyme Commission Summary:
The reaction also occurs with other medium-chain-length (C(6)-C(12)) hydroxyalkanoic acids.

The enzyme can also hydrolyze copolymers of medium-chain-length (MCL) hydroxyalkanoic acids and p-nitrophenyl esters of fatty acids, but short-chain-length hydroxyalkenoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolyzed.

There are two types of polymers: native (intracellular) granules are amorphous and have an intact surface layer; denatured (extracellular) granules either have no surface layer or a damaged surface layer and are partially crystalline.

Citations: [Jendrossek01, Garcia99, Schirmer93]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R05796

Relationship Links: BRENDA:EC:3.1.1.76 , ENZYME:EC:3.1.1.76 , IUBMB-ExplorEnz:EC:3.1.1.76


References

Garcia99: Garcia B, Olivera ER, Minambres B, Fernandez-Valverde M, Canedo LM, Prieto MA, Garcia JL, Martinez M, Luengo JM (1999). "Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon." J Biol Chem 274(41);29228-41. PMID: 10506180

Jendrossek01: Jendrossek D (2001). "Microbial degradation of polyesters." Adv Biochem Eng Biotechnol 71;293-325. PMID: 11217416

Schirmer93: Schirmer A, Jendrossek D, Schlegel HG (1993). "Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13." Appl Environ Microbiol 59(4);1220-7. PMID: 8476295


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, BIOCYC14A.