|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 188.8.131.52
Enzymes and Genes:
|Pseudomonas fluorescens GK13 :||extracellular poly(3-hydroxyoctanoate) depolymerase
|Pseudomonas putida KT2442 :||intracellular poly(3-hydroxyoctanoate) depolymerase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Mass balance status: Balance undetermined; a substrate lacks a chemical formula
Enzyme Commission Primary Name: poly(3-hydroxyoctanoate) depolymerase
Enzyme Commission Synonyms: PHO depolymerase, poly(3HO) depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly(HA) depolymerase, poly(HAMCL) depolymerase, poly[(R)-3-hydroxyoctanoate] hydrolase
Enzyme Commission Summary:
The reaction also occurs with other medium-chain-length (C(6)-C(12)) hydroxyalkanoic acids.
The enzyme can also hydrolyze copolymers of medium-chain-length (MCL) hydroxyalkanoic acids and p-nitrophenyl esters of fatty acids, but short-chain-length hydroxyalkenoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolyzed.
There are two types of polymers: native (intracellular) granules are amorphous and have an intact surface layer; denatured (extracellular) granules either have no surface layer or a damaged surface layer and are partially crystalline.
Unification Links: KEGG:R05796
Garcia99: Garcia B, Olivera ER, Minambres B, Fernandez-Valverde M, Canedo LM, Prieto MA, Garcia JL, Martinez M, Luengo JM (1999). "Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon." J Biol Chem 274(41);29228-41. PMID: 10506180
Schirmer93: Schirmer A, Jendrossek D, Schlegel HG (1993). "Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13." Appl Environ Microbiol 59(4);1220-7. PMID: 8476295
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