Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 3.1.1.76

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.1.1.76

Enzymes and Genes:
intracellular poly(3-hydroxyoctanoate) depolymerase Inferred from experiment : phaZ ( Pseudomonas putida KT2442 )
extracellular poly(3-hydroxyoctanoate) depolymerase Inferred from experiment : phaZ ( Pseudomonas fluorescens GK13 )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Undetermined; a substrate lacks a chemical formula

Enzyme Commission Primary Name: poly(3-hydroxyoctanoate) depolymerase

Enzyme Commission Synonyms: PHO depolymerase, poly(3HO) depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly(HA) depolymerase, poly(HAMCL) depolymerase, poly[(R)-3-hydroxyoctanoate] hydrolase

Enzyme Commission Summary:
The reaction also occurs with other medium-chain-length (C(6)-C(12)) hydroxyalkanoic acids.

The enzyme can also hydrolyze copolymers of medium-chain-length (MCL) hydroxyalkanoic acids and p-nitrophenyl esters of fatty acids, but short-chain-length hydroxyalkenoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolyzed.

There are two types of polymers: native (intracellular) granules are amorphous and have an intact surface layer; denatured (extracellular) granules either have no surface layer or a damaged surface layer and are partially crystalline.

Citations: [Jendrossek01, Garcia99, Schirmer93]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R05796

Relationship Links: BRENDA:EC:3.1.1.76 , ENZYME:EC:3.1.1.76 , IUBMB-ExplorEnz:EC:3.1.1.76


References

Garcia99: Garcia B, Olivera ER, Minambres B, Fernandez-Valverde M, Canedo LM, Prieto MA, Garcia JL, Martinez M, Luengo JM (1999). "Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon." J Biol Chem 274(41);29228-41. PMID: 10506180

Jendrossek01: Jendrossek D (2001). "Microbial degradation of polyesters." Adv Biochem Eng Biotechnol 71;293-325. PMID: 11217416

Schirmer93: Schirmer A, Jendrossek D, Schlegel HG (1993). "Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13." Appl Environ Microbiol 59(4);1220-7. PMID: 8476295


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.