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MetaCyc Reaction: 3.4.21.82

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.4.21.82

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: The left side is missing: Peptides: 2 . The right side is missing: Protein: 1 O: 1 H: 1

Enzyme Commission Primary Name: glutamyl endopeptidase II

Enzyme Commission Synonyms: GluSGP

Taxonomic Range: Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): 36.144756 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This enzyme catalyzes preferential cleavage: -Glu-|- >> -Asp-|- . Preference for Pro or Leu at P2 and Phe at P3. Cleavage of -Glu-|-Asp- and -Glu-|-Pro- bonds is slow.

From Streptomyces griseus. A peptidase of family S1 (trypsin family). Inhibited by [Leu18→Glu]-modified turkey ovomucoid third domain

Citations: [Yoshida88, Komiyama91, Nagata91, Svendsen91, Breddam92]

Relationship Links: BRENDA:EC:3.4.21.82 , ENZYME:EC:3.4.21.82 , IUBMB-ExplorEnz:EC:3.4.21.82 , UniProt:RELATED-TO:Q03424 , UniProt:RELATED-TO:Q54211


References

Breddam92: Breddam K, Meldal M (1992). "Substrate preferences of glutamic-acid-specific endopeptidases assessed by synthetic peptide substrates based on intramolecular fluorescence quenching." Eur J Biochem 206(1);103-7. PMID: 1587264

Komiyama91: Komiyama T, Bigler TL, Yoshida N, Noda K, Laskowski M (1991). "Replacement of P1 Leu18 by Glu18 in the reactive site of turkey ovomucoid third domain converts it into a strong inhibitor of Glu-specific Streptomyces griseus proteinase (GluSGP)." J Biol Chem 266(17);10727-30. PMID: 1674942

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Nagata91: Nagata K, Yoshida N, Ogata F, Araki M, Noda K (1991). "Subsite mapping of an acidic amino acid-specific endopeptidase from Streptomyces griseus, GluSGP, and protease V8." J Biochem 110(6);859-62. PMID: 1794975

Svendsen91: Svendsen I, Jensen MR, Breddam K (1991). "The primary structure of the glutamic acid-specific protease of Streptomyces griseus." FEBS Lett 292(1-2);165-7. PMID: 1959600

Yoshida88: Yoshida N, Tsuruyama S, Nagata K, Hirayama K, Noda K, Makisumi S (1988). "Purification and characterization of an acidic amino acid specific endopeptidase of Streptomyces griseus obtained from a commercial preparation (Pronase)." J Biochem 104(3);451-6. PMID: 3149277


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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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