|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 126.96.36.199
Supersedes EC numbers: 188.8.131.52, 184.108.40.206, 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Mass balance status: The left side is missing: Peptides: 2 . The right side is missing: Proteins: 1 O: 1 H: 1
Enzyme Commission Primary Name: fruit bromelain
Enzyme Commission Synonyms: ananase, bromelase, bromelin, extranase, juice bromelain, pinase, pineapple enzyme, traumanase, fruit bromelain FA2
Taxonomic Range: Viridiplantae
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): 36.144756 [Latendresse13]
Enzyme Commission Summary:
This enzyme catalyzes hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain). From the pineapple plant, Ananas comosus. Scarcely inhibited by chicken cystatin. Another cysteine endopeptidase, with similar action on small molecule substrates, pinguinain, is obtained from the related plant, Bromelia pinguin, but pinguinain differs from fruit bromelain in being inhibited by chicken cystatin[Rowan90].
Relationship Links: BRENDA:EC:18.104.22.168 , ENZYME:EC:22.214.171.124 , IUBMB-ExplorEnz:EC:126.96.36.199 , UniProt:RELATED-TO:O23791 , UniProt:RELATED-TO:O23800 , UniProt:RELATED-TO:O23801 , UniProt:RELATED-TO:O24641
Ota85: Ota S, Muta E, Katahira Y, Okamoto Y (1985). "Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains." J Biochem 98(1);219-28. PMID: 4044551
©2015 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493