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MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Supersedes EC numbers:,,

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: The left side is missing: Peptides: 2 . The right side is missing: Proteins: 1 O: 1 H: 1

Enzyme Commission Primary Name: fruit bromelain

Enzyme Commission Synonyms: ananase, bromelase, bromelin, extranase, juice bromelain, pinase, pineapple enzyme, traumanase, fruit bromelain FA2

Taxonomic Range: Viridiplantae

Standard Gibbs Free Energy (ΔrG in kcal/mol): 36.144756Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This enzyme catalyzes hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain). From the pineapple plant, Ananas comosus. Scarcely inhibited by chicken cystatin. Another cysteine endopeptidase, with similar action on small molecule substrates, pinguinain, is obtained from the related plant, Bromelia pinguin, but pinguinain differs from fruit bromelain in being inhibited by chicken cystatin [Rowan90].

Citations: [Sasaki73, Yamada76, Ota85]

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:O23791, UniProt:RELATED-TO:O23800, UniProt:RELATED-TO:O23801, UniProt:RELATED-TO:O24641


Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Ota85: Ota S, Muta E, Katahira Y, Okamoto Y (1985). "Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains." J Biochem 98(1);219-28. PMID: 4044551

Rowan90: Rowan AD, Buttle DJ, Barrett AJ (1990). "The cysteine proteinases of the pineapple plant." Biochem J 266(3);869-75. PMID: 2327970

Sasaki73: Sasaki M, Kato T, Iida S (1973). "Antigenic determinant common to four kinds of thiol proteases of plant origin." J Biochem 74(3);635-7. PMID: 4127920

Yamada76: Yamada F, Takahashi N, Murachi T (1976). "Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2." J Biochem 79(6);1223-34. PMID: 956152

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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