MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655: protease IIIInferred from experiment: ptrA

Supersedes EC number:

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balance undetermined; a substrate has a non-numerical coefficient

Enzyme Commission Primary Name: pitrilysin

Enzyme Commission Synonyms: Escherichia coli protease III, protease Pi, proteinase Pi, PTR, Escherichia coli metalloproteinase Pi

Taxonomic Range: Bacteria

Enzyme Commission Summary:
This enzyme catalyzes preferential cleavage of -Tyr16-|- Leu- and -Phe25-|- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon.

From the periplasmic space of Escherichia coli. Inhibited by EDTA and 1,10-phenanthroline; not thiol-dependent. Type example of peptidase family M16

Citations: [Finch86, Affholter88, Becker92, Ding92, Anastasi93, Cornista04, Cheng79]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:O48870, UniProt:RELATED-TO:P05458, UniProt:RELATED-TO:Q40983


Affholter88: Affholter JA, Fried VA, Roth RA (1988). "Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III." Science 242(4884);1415-8. PMID: 3059494

Anastasi93: Anastasi A, Knight CG, Barrett AJ (1993). "Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay." Biochem J 290 ( Pt 2);601-7. PMID: 7680857

Becker92: Becker AB, Roth RA (1992). "An unusual active site identified in a family of zinc metalloendopeptidases." Proc Natl Acad Sci U S A 89(9);3835-9. PMID: 1570301

Cheng79: Cheng YS, Zipser D (1979). "Purification and characterization of protease III from Escherichia coli." J Biol Chem 254(11);4698-706. PMID: 374413

Cornista04: Cornista J, Ikeuchi S, Haruki M, Kohara A, Takano K, Morikawa M, Kanaya S (2004). "Cleavage of various peptides with pitrilysin from Escherichia coli: kinetic analyses using beta-endorphin and its derivatives." Biosci Biotechnol Biochem 68(10);2128-37. PMID: 15502359

Ding92: Ding L, Becker AB, Suzuki A, Roth RA (1992). "Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III." J Biol Chem 267(4);2414-20. PMID: 1733942

Finch86: Finch PW, Wilson RE, Brown K, Hickson ID, Emmerson PT (1986). "Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III." Nucleic Acids Res 14(19);7695-703. PMID: 3534791

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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