Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 3.4.24.55

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.4.24.55

Enzymes and Genes:
protease III Inferred from experiment : ptrA ( Escherichia coli K-12 substr. MG1655 )

Supersedes EC number: 3.4.99.44

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Undetermined; a substrate lacks a chemical formula

Enzyme Commission Primary Name: pitrilysin

Enzyme Commission Synonyms: Escherichia coli protease III, protease Pi, proteinase Pi, PTR, Escherichia coli metalloproteinase Pi

Enzyme Commission Summary:
This enzyme catalyzes preferential cleavage of -Tyr16-|- Leu- and -Phe25-|- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon.

From the periplasmic space of Escherichia coli. Inhibited by EDTA and 1,10-phenanthroline; not thiol-dependent. Type example of peptidase family M16

Citations: [Finch86, Affholter88, Becker92, Ding92, Anastasi93, Cornista04, Cheng79]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:3.4.24.55 , ENZYME:EC:3.4.24.55 , IUBMB-ExplorEnz:EC:3.4.24.55 , UniProt:RELATED-TO:O48870 , UniProt:RELATED-TO:P05458 , UniProt:RELATED-TO:Q40983


References

Affholter88: Affholter JA, Fried VA, Roth RA (1988). "Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III." Science 242(4884);1415-8. PMID: 3059494

Anastasi93: Anastasi A, Knight CG, Barrett AJ (1993). "Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay." Biochem J 290 ( Pt 2);601-7. PMID: 7680857

Becker92: Becker AB, Roth RA (1992). "An unusual active site identified in a family of zinc metalloendopeptidases." Proc Natl Acad Sci U S A 89(9);3835-9. PMID: 1570301

Cheng79: Cheng YS, Zipser D (1979). "Purification and characterization of protease III from Escherichia coli." J Biol Chem 254(11);4698-706. PMID: 374413

Cornista04: Cornista J, Ikeuchi S, Haruki M, Kohara A, Takano K, Morikawa M, Kanaya S (2004). "Cleavage of various peptides with pitrilysin from Escherichia coli: kinetic analyses using beta-endorphin and its derivatives." Biosci Biotechnol Biochem 68(10);2128-37. PMID: 15502359

Ding92: Ding L, Becker AB, Suzuki A, Roth RA (1992). "Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III." J Biol Chem 267(4);2414-20. PMID: 1733942

Finch86: Finch PW, Wilson RE, Brown K, Hickson ID, Emmerson PT (1986). "Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III." Nucleic Acids Res 14(19);7695-703. PMID: 3534791


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC14B.