Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 3.4.24.60

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.4.24.60

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Undetermined; a substrate lacks a chemical formula

Enzyme Commission Primary Name: dactylysin

Enzyme Commission Synonyms: peptide hormone inactivating endopeptidase, PHIE

Enzyme Commission Summary:
This enzyme catalyzes hydrolysis of peptides of at least six residues, with bulky hydrophobic residues in the P1' position. Shows a preference for hydrophobic doublets such as -Phe-|-Phe- and -Phe-|-Leu- in somatostatin-(1-14)-peptide and dynorphin A-(1-6)-peptide, respectively.

An endopeptidase of 100 kDa secreted from the skin of the amphibian, Xenopus laevis (DactylÍtre du Cap). Resembles neprilysin in insensitivity to 1 M captopril, but differs from it in being insensitive to thiorphan (1 M) and unable to digest [Met5]enkephalin, [Leu5]enkephalin, oxytocin, and substance P-(7-11)-peptide. A similar endopeptidase is found in human neuroblastoma cells [Delporte92].

Citations: [Carvalho92, Joudiou93]

Relationship Links: BRENDA:EC:3.4.24.60 , ENZYME:EC:3.4.24.60 , IUBMB-ExplorEnz:EC:3.4.24.60


References

Carvalho92: Carvalho KM, Joudiou C, Boussetta H, Leseney AM, Cohen P (1992). "A peptide-hormone-inactivating endopeptidase in Xenopus laevis skin secretion." Proc Natl Acad Sci U S A 89(1);84-8. PMID: 1729723

Delporte92: Delporte C, Carvalho KM, Leseney AM, Winand J, Christophe J, Cohen P (1992). "A new metallo- endopeptidase from human neuroblastoma NB-OK-1 cells which inactivates atrial natriuretic peptide by selective cleavage at the Ser123-Phe124 bond." Biochem Biophys Res Commun 182(1);158-64. PMID: 1531011

Joudiou93: Joudiou C, Carvalho KM, Camarao G, Boussetta H, Cohen P (1993). "Characterization of the thermolysin-like cleavage of biologically active peptides by Xenopus laevis peptide hormone inactivating enzyme." Biochemistry 32(23);5959-66. PMID: 8507636


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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc14.