MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Populus alba : isoprene synthase Inferred from experiment : IspS

In Pathway: isoprene biosynthesis I , isoprene biosynthesis II (engineered)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: isoprene synthase

Enzyme Commission Synonyms: ISPC, ISPS

Standard Gibbs Free Energy (ΔrG in kcal/mol): -4.299988 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg(2+) concentration.

Citations: [Silver91, Wildermuth96, Miller01, Sivy02, Sasaki05, Schnitzler05, Silver95]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: KEGG:R08199 , Rhea:13369

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:


Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Miller01: Miller B, Oschinski C, Zimmer W (2001). "First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli." Planta 213(3);483-7. PMID: 11506373

Sasaki05: Sasaki K, Ohara K, Yazaki K (2005). "Gene expression and characterization of isoprene synthase from Populus alba." FEBS Lett 579(11);2514-8. PMID: 15848197

Schnitzler05: Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ (2005). "Biochemical properties of isoprene synthase in poplar (Populus x canescens)." Planta 222(5);777-86. PMID: 16052321

Silver91: Silver GM, Fall R (1991). "Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts." Plant Physiol 97(4);1588-91. PMID: 16668590

Silver95: Silver GM, Fall R (1995). "Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere." J Biol Chem 270(22);13010-6. PMID: 7768893

Sivy02: Sivy TL, Shirk MC, Fall R (2002). "Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis." Biochem Biophys Res Commun 294(1);71-5. PMID: 12054742

Wildermuth96: Wildermuth MC, Fall R (1996). "Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts)." Plant Physiol 112(1);171-182. PMID: 12226383

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat Aug 1, 2015, biocyc13.