|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → DNA-Reactions|
EC Number: 22.214.171.124
Enzymes and Genes:
|Escherichia coli K-12 substr. MG1655:||endonuclease III: nth|
Supersedes EC number: 126.96.36.199
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
Mass balance status: Balance undetermined; a substrate lacks a chemical formula
Enzyme Commission Primary Name: DNA-(apurinic or apyrimidinic site) lyase
Enzyme Commission Synonyms: AP lyase, AP endonuclease class I, endodeoxyribonuclease (apurinic or apyrimidinic), deoxyribonuclease (apurinic or apyrimidinic), E. coli endonuclease III, phage-T4 UV endonuclease, Micrococcus luteus UV endonuclease, AP site-DNA 5'-phosphomonoester-lyase, X-ray endonuclease III
Enzyme Commission Summary:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a β-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. This group of enzymes was previously listed as endonucleases, under EC 188.8.131.52.
Relationship Links: BRENDA:EC:184.108.40.206, ENZYME:EC:220.127.116.11, IUBMB-ExplorEnz:EC:18.104.22.168, UniProt:RELATED-TO:P04418, UniProt:RELATED-TO:P0AB83, UniProt:RELATED-TO:P22936, UniProt:RELATED-TO:P23196, UniProt:RELATED-TO:P27695, UniProt:RELATED-TO:P28352, UniProt:RELATED-TO:P43138, UniProt:RELATED-TO:P44319, UniProt:RELATED-TO:P50525, UniProt:RELATED-TO:P95945, UniProt:RELATED-TO:Q9CGM5, UniProt:RELATED-TO:Q9JVT0, UniProt:RELATED-TO:Q9PHS1, UniProt:RELATED-TO:Q9PNL1, UniProt:RELATED-TO:Q58829
Bailly89: Bailly V, Sente B, Verly WG (1989). "Bacteriophage-T4 and Micrococcus luteus UV endonucleases are not endonucleases but beta-elimination and sometimes beta delta-elimination catalysts." Biochem J 259(3);751-9. PMID: 2471512
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