|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 22.214.171.124
Enzymes and Genes:
|Eubacterium sp. A-44:||aryl-sulfate sulfotransferase: astA|
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
Mass balance status: Balanced.
Enzyme Commission Primary Name: aryl-sulfate sulfotransferase
Enzyme Commission Synonyms: arylsulfate-phenol sulfotransferase, arylsulfotransferase, ASST, arylsulfate sulfotransferase, arylsulfate:phenol sulfotransferase, astA (gene name)
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): 0.0 [Latendresse13]
Enzyme Commission Summary:
The enzyme, characterized from bacteria that colonize the human and mouse intestine, catalyses the transfer of a sulfate group from a phenol sulfate ester to other phenolic compounds. Activity is enhanced by Mg2+ and Mn2+ [Kim86]. Unlike EC 126.96.36.199, tyrosine-ester sulfotransferase and EC 188.8.131.52, aryl sulfotransferase, the enzyme does not act on 3'-phosphoadenylyl-sulfate or adenosine 3',5'-bisphosphate [Kim86]. The level of sulfation of polyphenols depends on the positions of the hydroxyl groups [Koizumi90, Koizumi91, KonishiImamura94]. Hydroxy groups of tyrosine residues in peptides such as angiotensin can also act as acceptors. The reaction proceeds according to a ping pong bi bi mechanism [Lee95a].
Kim86: Kim DH, Konishi L, Kobashi K (1986). "Purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine." Biochim Biophys Acta 872(1-2);33-41. PMID: 3460636
Koizumi91: Koizumi M, Akao T, Kadota S, Kikuchi T, Okuda T, Kobashi K (1991). "Enzymatic sulfation of polyphenols related to tannins by arylsulfotransferase." Chem Pharm Bull (Tokyo) 39(10);2638-43. PMID: 1806284
KonishiImamura94: Konishi-Imamura L, Sato M, Dohi K, Kadota S, Namba T, Kobashi K (1994). "Enzymatic sulfation of glycosides and their corresponding aglycones by arylsulfate sulfotransferase from a human intestinal bacterium." Biol Pharm Bull 17(8);1018-22. PMID: 7820100
Lee95a: Lee NS, Kim BT, Kim DH, Kobashi K (1995). "Purification and reaction mechanism of arylsulfate sulfotransferase from Haemophilus K-12, a mouse intestinal bacterium." J Biochem 118(4);796-801. PMID: 8576095
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