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MetaCyc Reaction: 2.3.1.86

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 2.3.1.86

Enzymes and Genes:

Saccharomyces cerevisiae : fatty acid synthase Inferred from experiment : FAS2 , FAS1

In Pathway: fatty acids biosynthesis (yeast)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Marked as unbalanced.

Enzyme Commission Primary Name: fatty-acyl-CoA synthase

Enzyme Commission Synonyms: yeast fatty acid synthase, FAS1 (gene name), FAS2 (gene name)

Enzyme Commission Summary:
The enzyme from yeasts (Ascomycota and Basidiomycota) is a multi-functional protein complex composed of two subunits. One subunit catalyses the reactions EC 1.1.1.100, 3-oxoacyl-[acyl-carrier-protein] reductase and EC 2.3.1.41, β-ketoacyl-[acyl-carrier-protein] synthase I, while the other subunit catalyses the reactions of EC 2.3.1.38, [acyl-carrier-protein] S-acetyltransferase, EC 2.3.1.39, [acyl-carrier-protein] S-malonyltransferase, EC 4.2.1.59, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, EC 1.3.1.10, enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific) and EC 1.1.1.279, (R)-3-hydroxyacid-ester dehydrogenase. The enzyme differs from the animal enzyme (EC 2.3.1.85) in that the enoyl reductase domain requires FMN as a cofactor, and the ultimate product is an acyl-CoA (usually palmitoyl-CoA) instead of a free fatty acid.

Citations: [Schweizer73, Wakil83, Tehlivets07]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reactions of [a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ ← a 3-oxoacyl-[acp] + NADPH + H+] (2.3.1.86):
i1: a (3R)-3-hydroxystearoyl-[acp] + NADP+ ← a 3-oxo-stearoyl-[acp] + NADPH + H+ (2.3.1.86)

i2: a (3R)-3-hydroxylignoceroyl-[acp] + NADP+ ← a 3-oxo-lignoceroyl-[acp] + NADPH + H+ (1.1.1.100)

i3: a (3R)-3-hydroxycerotoyl-[acp] + NADP+ ← a 3-oxo-cerotoyl-[acp] + NADPH + H+ (1.1.1.100)

i4: a (3R)-3-hydroxy-docosapentaenoyl-[acp] + NADP+ = a 3-oxo-docosapentaenoyl [acp] + NADPH + H+ (1.1.1.100/2.3.1.85/2.3.1.86)

i5: a (3R)-3-hydroxymyristoyl-[acp] + NADP+ ← a 3-oxo-myristoyl-[acp] + NADPH + H+ (2.3.1.86)

i6: a (3R)-3-hydroxydodecanoyl-[acp] + NADP+ ← a 3-oxo-dodecanoyl-[acp] + NADPH + H+ (2.3.1.86)

i7: a (3R)-3-hydroxyoctanoyl-[acp] + NADP+ ← a 3-oxo-octanoyl-[acp] + NADPH + H+ (2.3.1.86)

i8: a (3R)-3-hydroxyarachidoyl-[acp] + NADP+ ← a 3-oxo-arachidoyl-[acp] + NADPH + H+ (1.1.1.100)

i9: a (3R)-3-hydroxydecanoyl-[acp] + NADP+ ← a 3-oxo-decanoyl-[acp] + NADPH + H+ (2.3.1.86)

i10: a (3R)-3-hydroxyhexanoyl-[acp] + NADP+ ← a 3-oxo-hexanoyl-[acp] + NADPH + H+ (2.3.1.86)

i11: a (3R)-3-hydroxybehenoyl-[acp] + NADP+ = a 3-oxo-behenoyl-[acp] + NADPH + H+ (1.1.1.100)

i12: a (3R)-3-hydroxypalmitoyl-[acp] + NADP+ ← a 3-oxo-palmitoyl-[acp] + NADPH + H+ (2.3.1.86)

Instance reaction of [a (3R)-3-hydroxyacyl-[acyl-carrier protein] → a trans-2-enoyl-[acyl-carrier protein] + H2O] (2.3.1.86/4.2.1.59):
i13: a (3R)-3-hydroxymyristoyl-[acp] → a trans tetradec-2-enoyl-[acp] + H2O (2.3.1.86/4.2.1.59)

Instance reactions of [a 2,3,4-saturated fatty acyl-[acp] + NADP+ ← a trans-2-enoyl-[acyl-carrier protein] + NADPH + H+] (1.3.1.39/1.3.1.104/2.3.1.85/2.3.1.86):
i14: an octanoyl-[acp] + NADP+ ← a trans oct-2-enoyl-[acp] + NADPH + H+ (1.3.1.10/1.3.1.39/2.3.1.85/2.3.1.86)

i15: a dodecanoyl-[acp] + NADP+ ← a (2E)-dodec-2-enoyl-[acp] + NADPH + H+ (1.3.1.10/1.3.1.39/2.3.1.85/2.3.1.86)

i16: a stearoyl-[acp] + NADP+ ← a trans-octadec-2-enoyl-[acp] + NADPH + H+ (1.3.1.10/2.3.1.86)

i17: a palmitoyl-[acp] + NADP+ ← a trans hexadecenoyl-[acp] + NADPH + H+ (1.3.1.10/1.3.1.39/2.3.1.85/2.3.1.86)

i18: a myristoyl-[acp] + NADP+ ← a trans tetradec-2-enoyl-[acp] + NADPH + H+ (1.3.1.10/1.3.1.39/2.3.1.85/2.3.1.86)

i19: a hexanoyl-[acyl-carrier-protein] + NADP+ ← a trans hex-2-enoyl-[acp] + NADPH + H+ (1.3.1.10/1.3.1.39/2.3.1.85/2.3.1.86)

i20: a decanoyl-[acp] + NADP+ ← a (2E)-dec-2-enoyl-[acp] + NADPH + H+ (1.3.1.10/1.3.1.39/2.3.1.85/2.3.1.86)

Unification Links: KEGG:R05190

Relationship Links: BRENDA:EC:2.3.1.86 , ENZYME:EC:2.3.1.86 , IUBMB-ExplorEnz:EC:2.3.1.86 , UniProt:RELATED-TO:P07149 , UniProt:RELATED-TO:P34229 , UniProt:RELATED-TO:P34731 , UniProt:RELATED-TO:Q9UUG0

Credits:
Revised 27-Sep-2012 by Caspi R , SRI International


References

Schweizer73: Schweizer E, Kniep B, Castorph H, Holzner U (1973). "Pantetheine-free mutants of the yeast fatty-acid-synthetase complex." Eur J Biochem 39(2);353-62. PMID: 4590449

Tehlivets07: Tehlivets O, Scheuringer K, Kohlwein SD (2007). "Fatty acid synthesis and elongation in yeast." Biochim Biophys Acta 1771(3);255-70. PMID: 16950653

Wakil83: Wakil SJ, Stoops JK, Joshi VC (1983). "Fatty acid synthesis and its regulation." Annu Rev Biochem 52;537-79. PMID: 6137188


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Aug 4, 2015, biocyc11.