MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: ,

Enzymes and Genes:

Arabidopsis thaliana col : purple acid phosphatase [multifunctional] Inferred from experiment : PAP26
Escherichia coli K-12 substr. MG1655 : alkaline phosphatase Inferred from experiment : phoA
sugar phosphatase : ybiV
sugar phosphatase : yidA
phosphosugar phosphatase : yigL
sugar phosphatase : yfbT
Homo sapiens : glucose-6-phosphatase Inferred from experiment : G6PC
glucose-6-phosphatase 3 Inferred from experiment : G6PC3
glucose-6-phosphatase 2 Inferred from experiment : G6PC2

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

Reaction Locations: cytosol, periplasmic space (sensu Gram-negative Bacteria)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Direct generic reaction:
D-glucopyranose 6-phosphate + H2O → D-glucopyranose + phosphate (

Enzyme Commission Primary Name for glucose-6-phosphatase

Enzyme Commission Synonyms for glucose 6-phosphate phosphatase

Enzyme Commission Primary Name for sugar-terminal-phosphatase

Enzyme Commission Synonyms for xylitol-5-phosphatase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.2170105 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary for
Wide distribution in animal tissues. Also catalyses potent transphosphorylations from carbamoyl phosphate, hexose phosphates, diphosphate, phosphoenolpyruvate and nucleoside di- and triphosphates, to D-glucose, D-mannose, 3-methyl-D-glucose or 2-deoxy-D-glucose [cf. EC (phosphoramidate—hexose phosphotransferase}, EC (diphosphate—glycerol phosphotransferase) and EC (phosphoamidase)].

Enzyme Commission Summary for
Acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, e.g. good substrates are glucose 6-phosphate, mannose 6-phosphate, 6-phosphogluconate, erythrose 4-phosphate and xylitol 5-phosphate.

Citations: [Anchors75, Colilla75, Nordlie74, London85]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reaction of [D-glucopyranose 6-phosphate + H2O → D-glucopyranose + phosphate] (
i1: β-D-glucose 6-phosphate + H2O → β-D-glucose + phosphate (

Relationship Links: BRENDA:EC: , BRENDA:EC: , ENZYME:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:P35575 , UniProt:RELATED-TO:P35576 , UniProt:RELATED-TO:P43428

Revised 06-Apr-2011 by Caspi R , SRI International


Anchors75: Anchors JM, Karnovsky ML (1975). "Purification of cerebral glucose-6-phosphatase. An enzyme involved in sleep." J Biol Chem 250(16);6408-16. PMID: 169241

Colilla75: Colilla W, Jorgenson RA, Nordlie RC (1975). "Mammalian carbamyl phosphate : glucose phosphotransferase and glucose-6-phosphate phosphohydrolase: extended tissue distribution." Biochim Biophys Acta 377(1);117-25. PMID: 164220

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

London85: London J, Hausman SZ, Thompson J (1985). "Characterization of a membrane-regulated sugar phosphate phosphohydrolase from Lactobacillus casei." J Bacteriol 163(3);951-6. PMID: 2993253

Nordlie74: Nordlie RC (1974). "Metabolic regulation by multifunctional glucose-6-phosphatase." Curr Top Cell Regul 8(0);33-117. PMID: 4370737

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Apr 20, 2015, biocyc13.