MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Arabidopsis thaliana col: glucosamine:fructose-6-phosphate aminotransferaseInferred from experimentInferred by computational analysis: GFAT
Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819: D-glucosamine-6-phosphate synthaseInferred from experiment: ptmF, ptmA
Candida albicans: glucosamine-6-phosphate synthaseInferred from experiment: GFA1
Drosophila melanogaster: glucosamine-fructose-6-phosphate aminotransferaseInferred from experiment: Gfat1
Escherichia coli K-12 substr. MG1655: L-glutamine:D-fructose-6-phosphate aminotransferaseInferred from experiment: glmS
Homo sapiens: glutamine:fructose-6-phosphate amidotransferase IInferred from experiment: GFPT1
Rattus norvegicus: glutamine:fructose-6-phosphate amidotransferaseInferred from experiment: Gfpt1

In Pathway: UDP-N-acetyl-D-glucosamine biosynthesis II, CMP-legionaminate biosynthesis I, UDP-N-acetyl-D-glucosamine biosynthesis I

Supersedes EC number:

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: glutamine—fructose-6-phosphate transaminase (isomerizing)

Enzyme Commission Synonyms: hexosephosphate aminotransferase, glucosamine-6-phosphate isomerase (glutamine-forming), glutamine-fructose-6-phosphate transaminase (isomerizing), D-fructose-6-phosphate amidotransferase, glucosaminephosphate isomerase, glucosamine 6-phosphate synthase, GlcN6P synthase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -3.4358826Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Although the overall reaction is that of a transferase, the mechanism involves the formation of ketimine between fructose 6-phosphate and a 6-amino group from a lysine residue at the active site, which is subsequently displaced by ammonia (transamidination).

Citations: [Teplyakov99, Leloir53, Gryder60, Ghosh60]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R00768, Rhea:13237

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:O19908, UniProt:RELATED-TO:O26060, UniProt:RELATED-TO:O26273, UniProt:RELATED-TO:O66648, UniProt:RELATED-TO:O83833, UniProt:RELATED-TO:O84823, UniProt:RELATED-TO:O86781, UniProt:RELATED-TO:P08633, UniProt:RELATED-TO:P0A588, UniProt:RELATED-TO:P14742, UniProt:RELATED-TO:P17169, UniProt:RELATED-TO:P25195, UniProt:RELATED-TO:P39754, UniProt:RELATED-TO:P40831, UniProt:RELATED-TO:P44708, UniProt:RELATED-TO:P47856, UniProt:RELATED-TO:P53704, UniProt:RELATED-TO:P72720, UniProt:RELATED-TO:Q9JWN9, UniProt:RELATED-TO:Q9K1P9, UniProt:RELATED-TO:Q9PLA4, UniProt:RELATED-TO:Q9PMT4, UniProt:RELATED-TO:Q9RXK9, UniProt:RELATED-TO:Q9S168, UniProt:RELATED-TO:Q9V249, UniProt:RELATED-TO:Q9WXZ5, UniProt:RELATED-TO:Q9Z6U0, UniProt:RELATED-TO:Q9ZJ94, UniProt:RELATED-TO:Q06210, UniProt:RELATED-TO:Q09740, UniProt:RELATED-TO:Q19130, UniProt:RELATED-TO:Q19699, UniProt:RELATED-TO:Q56206, UniProt:RELATED-TO:Q56275, UniProt:RELATED-TO:Q58815, UniProt:RELATED-TO:Q84421


Ghosh60: Ghosh S, Blumenthal HJ, Davidson E, Roseman S (1960). "Glucosamine metabolism. V. Enzymatic synthesis of glucosamine 6-phosphate." J Biol Chem 235;1265-73. PMID: 13827775

Gryder60: Gryder RM, Pogell BM (1960). "Further studies on glucosamine 6-phosphate synthesis by rat liver enzymes." J Biol Chem 235;558-62. PMID: 13829889

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Leloir53: Leloir LF, Cardini CE (1953). "The biosynthesis of glucosamine." Biochim Biophys Acta 12(1-2);15-22. PMID: 13115409

Teplyakov99: Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B (1999). "The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase." Protein Sci 8(3);596-602. PMID: 10091662

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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