Metabolic Modeling Tutorial
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BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Reaction: 2.6.1.16

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 2.6.1.16

Enzymes and Genes:
L-glutamine:D-fructose-6-phosphate aminotransferase Inferred from experiment : glmS ( Escherichia coli K-12 substr. MG1655 )
glucosamine:fructose-6-phosphate aminotransferase Inferred from experiment Inferred by computational analysis : GFAT ( Arabidopsis thaliana col )
glucosamine-fructose-6-phosphate aminotransferase Inferred from experiment : Gfat1 ( Drosophila melanogaster )
D-glucosamine-6-phosphate synthase Inferred from experiment : ptmF , ptmA ( Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819 )
glucosamine-6-phosphate synthase Inferred from experiment : GFA1 ( Candida albicans )
glutamine:fructose-6-phosphate amidotransferase I Inferred from experiment : GFPT1 ( Homo sapiens )
glutamine:fructose-6-phosphate amidotransferase Inferred from experiment : Gfpt1 ( Rattus norvegicus )

In Pathway: UDP-N-acetyl-D-glucosamine biosynthesis II , CMP-legionaminate biosynthesis I , UDP-N-acetyl-D-glucosamine biosynthesis I

Supersedes EC number: 5.3.1.19

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: glutamine—fructose-6-phosphate transaminase (isomerizing)

Enzyme Commission Synonyms: hexosephosphate aminotransferase, glucosamine-6-phosphate isomerase (glutamine-forming), glutamine-fructose-6-phosphate transaminase (isomerizing), D-fructose-6-phosphate amidotransferase, glucosaminephosphate isomerase, glucosamine 6-phosphate synthase, GlcN6P synthase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -3.4358826 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Although the overall reaction is that of a transferase, the mechanism involves the formation of ketimine between fructose 6-phosphate and a 6-amino group from a lysine residue at the active site, which is subsequently displaced by ammonia (transamidination).

Citations: [Teplyakov99, LELOIR, GRYDER60, GHOSH60]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R00768 , Rhea:13237

Relationship Links: BRENDA:EC:2.6.1.16 , ENZYME:EC:2.6.1.16 , IUBMB-ExplorEnz:EC:2.6.1.16 , UniProt:RELATED-TO:O19908 , UniProt:RELATED-TO:O26060 , UniProt:RELATED-TO:O26273 , UniProt:RELATED-TO:O66648 , UniProt:RELATED-TO:O83833 , UniProt:RELATED-TO:O84823 , UniProt:RELATED-TO:O86781 , UniProt:RELATED-TO:P08633 , UniProt:RELATED-TO:P0A588 , UniProt:RELATED-TO:P14742 , UniProt:RELATED-TO:P17169 , UniProt:RELATED-TO:P25195 , UniProt:RELATED-TO:P39754 , UniProt:RELATED-TO:P40831 , UniProt:RELATED-TO:P44708 , UniProt:RELATED-TO:P47856 , UniProt:RELATED-TO:P53704 , UniProt:RELATED-TO:P72720 , UniProt:RELATED-TO:Q9JWN9 , UniProt:RELATED-TO:Q9K1P9 , UniProt:RELATED-TO:Q9PLA4 , UniProt:RELATED-TO:Q9PMT4 , UniProt:RELATED-TO:Q9RXK9 , UniProt:RELATED-TO:Q9S168 , UniProt:RELATED-TO:Q9V249 , UniProt:RELATED-TO:Q9WXZ5 , UniProt:RELATED-TO:Q9Z6U0 , UniProt:RELATED-TO:Q9ZJ94 , UniProt:RELATED-TO:Q06210 , UniProt:RELATED-TO:Q09740 , UniProt:RELATED-TO:Q19130 , UniProt:RELATED-TO:Q19699 , UniProt:RELATED-TO:Q56206 , UniProt:RELATED-TO:Q56275 , UniProt:RELATED-TO:Q58815 , UniProt:RELATED-TO:Q84421


References

GHOSH60: GHOSH S, BLUMENTHAL HJ, DAVIDSON E, ROSEMAN S (1960). "Glucosamine metabolism. V. Enzymatic synthesis of glucosamine 6-phosphate." J Biol Chem 235;1265-73. PMID: 13827775

GRYDER60: GRYDER RM, POGELL BM (1960). "Further studies on glucosamine 6-phosphate synthesis by rat liver enzymes." J Biol Chem 235;558-62. PMID: 13829889

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

LELOIR: LELOIR LF, CARDINI CE "The biosynthesis of glucosamine." Biochim Biophys Acta 12(1-2);15-22. PMID: 13115409

Teplyakov99: Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B (1999). "The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase." Protein Sci 8(3);596-602. PMID: 10091662


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.