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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 2.6.1.16

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 2.6.1.16

Enzymes and Genes:
L-glutamine:D-fructose-6-phosphate aminotransferase Inferred from experiment : glmS ( Escherichia coli K-12 substr. MG1655 )
glucosamine:fructose-6-phosphate aminotransferase Inferred from experiment Inferred by computational analysis : GFAT ( Arabidopsis thaliana col )
glucosamine-fructose-6-phosphate aminotransferase Inferred from experiment : Gfat1 ( Drosophila melanogaster )
D-glucosamine-6-phosphate synthase Inferred from experiment : ptmF , ptmA ( Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819 )
glucosamine-6-phosphate synthase Inferred from experiment : GFA1 ( Candida albicans )
glutamine:fructose-6-phosphate amidotransferase I Inferred from experiment : GFPT1 ( Homo sapiens )
glutamine:fructose-6-phosphate amidotransferase Inferred from experiment : Gfpt1 ( Rattus norvegicus )

In Pathway: UDP-N-acetyl-D-glucosamine biosynthesis II , CMP-legionaminate biosynthesis I , UDP-N-acetyl-D-glucosamine biosynthesis I

Supersedes EC number: 5.3.1.19

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: glutamine—fructose-6-phosphate transaminase (isomerizing)

Enzyme Commission Synonyms: hexosephosphate aminotransferase, glucosamine-6-phosphate isomerase (glutamine-forming), glutamine-fructose-6-phosphate transaminase (isomerizing), D-fructose-6-phosphate amidotransferase, glucosaminephosphate isomerase, glucosamine 6-phosphate synthase, GlcN6P synthase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -3.4358826 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Although the overall reaction is that of a transferase, the mechanism involves the formation of ketimine between fructose 6-phosphate and a 6-amino group from a lysine residue at the active site, which is subsequently displaced by ammonia (transamidination).

Citations: [Teplyakov99, LELOIR, GRYDER60, GHOSH60]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R00768 , Rhea:13237

Relationship Links: BRENDA:EC:2.6.1.16 , ENZYME:EC:2.6.1.16 , IUBMB-ExplorEnz:EC:2.6.1.16 , UniProt:RELATED-TO:O19908 , UniProt:RELATED-TO:O26060 , UniProt:RELATED-TO:O26273 , UniProt:RELATED-TO:O66648 , UniProt:RELATED-TO:O83833 , UniProt:RELATED-TO:O84823 , UniProt:RELATED-TO:O86781 , UniProt:RELATED-TO:P08633 , UniProt:RELATED-TO:P0A588 , UniProt:RELATED-TO:P14742 , UniProt:RELATED-TO:P17169 , UniProt:RELATED-TO:P25195 , UniProt:RELATED-TO:P39754 , UniProt:RELATED-TO:P40831 , UniProt:RELATED-TO:P44708 , UniProt:RELATED-TO:P47856 , UniProt:RELATED-TO:P53704 , UniProt:RELATED-TO:P72720 , UniProt:RELATED-TO:Q9JWN9 , UniProt:RELATED-TO:Q9K1P9 , UniProt:RELATED-TO:Q9PLA4 , UniProt:RELATED-TO:Q9PMT4 , UniProt:RELATED-TO:Q9RXK9 , UniProt:RELATED-TO:Q9S168 , UniProt:RELATED-TO:Q9V249 , UniProt:RELATED-TO:Q9WXZ5 , UniProt:RELATED-TO:Q9Z6U0 , UniProt:RELATED-TO:Q9ZJ94 , UniProt:RELATED-TO:Q06210 , UniProt:RELATED-TO:Q09740 , UniProt:RELATED-TO:Q19130 , UniProt:RELATED-TO:Q19699 , UniProt:RELATED-TO:Q56206 , UniProt:RELATED-TO:Q56275 , UniProt:RELATED-TO:Q58815 , UniProt:RELATED-TO:Q84421


References

GHOSH60: GHOSH S, BLUMENTHAL HJ, DAVIDSON E, ROSEMAN S (1960). "Glucosamine metabolism. V. Enzymatic synthesis of glucosamine 6-phosphate." J Biol Chem 235;1265-73. PMID: 13827775

GRYDER60: GRYDER RM, POGELL BM (1960). "Further studies on glucosamine 6-phosphate synthesis by rat liver enzymes." J Biol Chem 235;558-62. PMID: 13829889

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

LELOIR: LELOIR LF, CARDINI CE "The biosynthesis of glucosamine." Biochim Biophys Acta 12(1-2);15-22. PMID: 13115409

Teplyakov99: Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B (1999). "The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase." Protein Sci 8(3);596-602. PMID: 10091662


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14A.