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MetaCyc Reaction: 2.1.1.192

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-Reactions

EC Number: 2.1.1.192

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655: 23S rRNA m2A2503 methyltransferase and tRNA m2A37 methyltransferaseInferred from experiment: rlmN

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 23S rRNA (adenine2503-C2)-methyltransferase

Enzyme Commission Synonyms: RlmN, YfgB, Cfr

Taxonomic Range: Archaea, Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): -51.300446Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Contains an [4Fe-4S] cluster [Yan10]. RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis [Yan10]. The enzyme methylates adenosine by a radical mechanism with CH2 from the S-adenosyl-L-methionine and retention of the hydrogen at C-2 of adenosine2503 of 23S rRNA. It will also methylate 8-methyladenosine2503 of 23S rRNA. This enzyme is a member of the 'AdoMet radical ' (radical SAM) family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group.

Citations: [Toh08, Yan11, Grove11, Boal11]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:2.1.1.192, ENZYME:EC:2.1.1.192, IUBMB-ExplorEnz:EC:2.1.1.192

Credits:
Created 10-Aug-2010 by Kothari A, SRI International
Revised 19-Sep-2011 by Caspi R, SRI International


References

Boal11: Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC (2011). "Structural basis for methyl transfer by a radical SAM enzyme." Science 332(6033);1089-92. PMID: 21527678

Grove11: Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases." Science 332(6029);604-7. PMID: 21415317

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Toh08: Toh SM, Xiong L, Bae T, Mankin AS (2008). "The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA." RNA 14(1);98-106. PMID: 18025251

Yan10: Yan F, LaMarre JM, Rohrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA." J Am Chem Soc 132(11);3953-64. PMID: 20184321

Yan11: Yan F, Fujimori DG (2011). "RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift." Proc Natl Acad Sci U S A 108(10);3930-4. PMID: 21368151


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Mon Feb 8, 2016, biocyc12.