Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015

MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655 : 23S rRNA m2A2503 methyltransferase and tRNA m2A37 methyltransferase Inferred from experiment : rlmN

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 23S rRNA (adenine2503-C2)-methyltransferase

Enzyme Commission Synonyms: RlmN, YfgB, Cfr

Taxonomic Range: Archaea , Bacteria

Standard Gibbs Free Energy (ΔrG in kcal/mol): -51.300446 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Contains an [4Fe-4S] cluster [Yan10]. RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis [Yan10]. The enzyme methylates adenosine by a radical mechanism with CH2 from the S-adenosyl-L-methionine and retention of the hydrogen at C-2 of adenosine2503 of 23S rRNA. It will also methylate 8-methyladenosine2503 of 23S rRNA. This enzyme is a member of the 'AdoMet radical ' (radical SAM) family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group.

Citations: [Toh08, Yan11, Grove11, Boal11]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Created 10-Aug-2010 by Kothari A , SRI International
Revised 19-Sep-2011 by Caspi R , SRI International


Boal11: Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC (2011). "Structural basis for methyl transfer by a radical SAM enzyme." Science 332(6033);1089-92. PMID: 21527678

Grove11: Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases." Science 332(6029);604-7. PMID: 21415317

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Toh08: Toh SM, Xiong L, Bae T, Mankin AS (2008). "The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA." RNA 14(1);98-106. PMID: 18025251

Yan10: Yan F, LaMarre JM, Rohrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA." J Am Chem Soc 132(11);3953-64. PMID: 20184321

Yan11: Yan F, Fujimori DG (2011). "RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift." Proc Natl Acad Sci U S A 108(10);3930-4. PMID: 21368151

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Jun 30, 2015, BIOCYC14B.