|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions|
EC Number: 188.8.131.52
Enzymes and Genes:
23S rRNA m2A2503 methyltransferase and tRNA m2A37 methyltransferase : rlmN ( Escherichia coli K-12 substr. MG1655 )
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Mass balance status: Balanced.
Enzyme Commission Primary Name: 23S rRNA (adenine2503-C2)-methyltransferase
Enzyme Commission Synonyms: RlmN, YfgB, Cfr
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -49.4281 [Latendresse13]
Enzyme Commission Summary:
Contains an [4Fe-4S] cluster [Yan10]. RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis [Yan10]. The enzyme methylates adenosine by a radical mechanism with CH2 from the S-adenosyl-L-methionine and retention of the hydrogen at C-2 of adenosine2503 of 23S rRNA. It will also methylate 8-methyladenosine2503 of 23S rRNA. This enzyme is a member of the 'AdoMet radical ' (radical SAM) family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group.
Grove11: Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases." Science 332(6029);604-7. PMID: 21415317
Yan10: Yan F, LaMarre JM, Rohrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA." J Am Chem Soc 132(11);3953-64. PMID: 20184321
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