Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015

MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: ,

Enzymes and Genes:
hydroperoxidase I Inferred from experiment : katG ( Escherichia coli K-12 substr. MG1655 )
versatile peroxidase VPL1 Inferred from experiment : vpl1 ( Pleurotus eryngii )
versatile peroxidase VPL2 Inferred from experiment : vpl2 ( Pleurotus eryngii )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name for versatile peroxidase

Enzyme Commission Synonyms for VP, hybrid peroxidase, polyvalent peroxidase

Enzyme Commission Primary Name for catalase-peroxidase

Enzyme Commission Synonyms for katG (gene name)

Enzyme Commission Summary for
This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC, manganese peroxidase and EC, lignin peroxidase. It is also able to oxidize phenols, hydroquinones and both low- and high redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [Heinfling98, Camarero99].

Enzyme Commission Summary for
Differs from EC, peroxidase in having a relatively high catalase (EC activity with hydrogen peroxide as donor, releasing oxygen; both activities use the same heme active site. In Mycobacterium tuberculosis it is responsible for activation of the commonly used antitubercular drug, isoniazid.

Citations: [Martinez96, RuizDuenas99, RuizDuenas99a, Camarero00, RuizDuenas01, Banci03, PerezBoada05, Caramelo99, Loewen85, Hochman91, Fraaije96, Bertrand04, Vlasits10]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R03532

Relationship Links: BRENDA:EC: , BRENDA:EC: , ENZYME:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , IUBMB-ExplorEnz:EC:

Revised 13-Sep-2012 by Caspi R , SRI International


Banci03: Banci L, Camarero S, Martinez AT, Martinez MJ, Perez-Boada M, Pierattelli R, Ruiz-Duenas FJ (2003). "NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii." J Biol Inorg Chem 8(7);751-60. PMID: 12884090

Bertrand04: Bertrand T, Eady NA, Jones JN, Jesmin , Nagy JM, Jamart-Gregoire B, Raven EL, Brown KA (2004). "Crystal structure of Mycobacterium tuberculosis catalase-peroxidase." J Biol Chem 279(37);38991-9. PMID: 15231843

Camarero00: Camarero S, Ruiz-Duenas FJ, Sarkar S, Martinez MJ, Martinez AT (2000). "The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases." FEMS Microbiol Lett 191(1);37-43. PMID: 11004397

Camarero99: Camarero S, Sarkar S, Ruiz-Duenas FJ, Martinez MJ, Martinez AT (1999). "Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites." J Biol Chem 274(15);10324-30. PMID: 10187820

Caramelo99: Caramelo L, Martinez MJ, Martinez AT (1999). "A search for ligninolytic peroxidases in the fungus pleurotus eryngii involving alpha-keto-gamma-thiomethylbutyric acid and lignin model dimers." Appl Environ Microbiol 65(3);916-22. PMID: 10049842

Fraaije96: Fraaije MW, Roubroeks HP, Hagen WR, Van Berkel WJ (1996). "Purification and characterization of an intracellular catalase-peroxidase from Penicillium simplicissimum." Eur J Biochem 235(1-2);192-8. PMID: 8631329

Heinfling98: Heinfling A, Ruiz-Duenas FJ, Martinez MJ, Bergbauer M, Szewzyk U, Martinez AT (1998). "A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta." FEBS Lett 428(3);141-6. PMID: 9654123

Hochman91: Hochman A, Goldberg I (1991). "Purification and characterization of a catalase-peroxidase and a typical catalase from the bacterium Klebsiella pneumoniae." Biochim Biophys Acta 1077(3);299-307. PMID: 2029529

Loewen85: Loewen PC, Triggs BL, George CS, Hrabarchuk BE (1985). "Genetic mapping of katG, a locus that affects synthesis of the bifunctional catalase-peroxidase hydroperoxidase I in Escherichia coli." J Bacteriol 1985;162(2);661-7. PMID: 3886630

Martinez96: Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT (1996). "Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii." Eur J Biochem 237(2);424-32. PMID: 8647081

PerezBoada05: Perez-Boada M, Ruiz-Duenas FJ, Pogni R, Basosi R, Choinowski T, Martinez MJ, Piontek K, Martinez AT (2005). "Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways." J Mol Biol 354(2);385-402. PMID: 16246366

RuizDuenas01: Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martinez MJ, Martinez AT (2001). "A new versatile peroxidase from Pleurotus." Biochem Soc Trans 29(Pt 2);116-22. PMID: 11356138

RuizDuenas99: Ruiz-Duenas FJ, Martinez MJ, Martinez AT (1999). "Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii." Mol Microbiol 31(1);223-35. PMID: 9987124

RuizDuenas99a: Ruiz-Duenas FJ, Martinez MJ, Martinez AT (1999). "Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates." Appl Environ Microbiol 65(10);4705-7. PMID: 10508113

Vlasits10: Vlasits J, Jakopitsch C, Bernroitner M, Zamocky M, Furtmuller PG, Obinger C (2010). "Mechanisms of catalase activity of heme peroxidases." Arch Biochem Biophys 500(1);74-81. PMID: 20434429

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Feb 26, 2015, biocyc13.