MetaCyc Reaction: [no EC number assigned]

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

Enzymes and Genes:

Arabidopsis thaliana col: 15-cis-phytoene desaturaseInferred from experiment: PDS
Synechococcus elongatus PCC 7942: 15-cis phytoene desaturaseInferred from experiment: pds

Sub-reaction of: 15-cis-phytoene + 2 a plastoquinone = 9,15,9'-tri-cis-ζ-carotene + 2 a plastoquinol

In Pathway: trans-lycopene biosynthesis II (plants)

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Standard Gibbs Free Energy (ΔrG in kcal/mol): 24.23999Inferred by computational analysis [Latendresse13]

This enzyme is involved in carotenoid biosynthesis in plants and cyanobacteria. The enzyme from Synechococcus can also use NAD+ and NADP+ as electron acceptor under anaerobic conditions. The enzyme from Gentiana lutea shows no activity with NAD+ or NADP+ [Breitenbach01].

This is one of two reactions catalyzed successively by the enzyme. The overall reaction is

15-cis-phytoene + 2 a plastoquinone = 9,15,9'-tri-cis-ζ-carotene + 2 a plastoquinol

Citations: [Schneider97c, Fraser93, Breitenbach05]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R09653, Rhea:30194

Created 17-Mar-2011 by Caspi R, SRI International


Breitenbach01: Breitenbach J, Zhu C, Sandmann G (2001). "Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors." J Agric Food Chem 49(11);5270-2. PMID: 11714315

Breitenbach05: Breitenbach J, Sandmann G (2005). "zeta-Carotene cis isomers as products and substrates in the plant poly-cis carotenoid biosynthetic pathway to lycopene." Planta 220(5);785-93. PMID: 15503129

Fraser93: Fraser PD, Linden H, Sandmann G (1993). "Purification and reactivation of recombinant Synechococcus phytoene desaturase from an overexpressing strain of Escherichia coli." Biochem J 291 ( Pt 3);687-92. PMID: 8489496

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Schneider97c: Schneider C, Boger P, Sandmann G (1997). "Phytoene desaturase: heterologous expression in an active state, purification, and biochemical properties." Protein Expr Purif 10(2);175-9. PMID: 9226712

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Wed Nov 25, 2015, biocyc12.