MetaCyc Reaction: [no EC number assigned]

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

Sub-reaction of: 2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Standard Gibbs Free Energy (ΔrG in kcal/mol): -3.6599884 Inferred by computational analysis [Latendresse13]

Requires Mn2+ for activity. The enzyme appears to be stereospecific, normally producing 15-cis-phytoene. However, in Pantoea agglomerans (Erwinia herbicola), the product is the 15-trans isomer [IwataReuyl03].

Citations: [Gregonis74, Misawa94]

Created 17-Mar-2011 by Caspi R , SRI International


Gregonis74: Gregonis DE, Rilling HC (1974). "The stereochemistry of trans-phytoene synthesis. Some observations on lycopersene as a carotene precursor and a mechanism for the synthesis of cis- and trans-phytoene." Biochemistry 13(7);1538-42. PMID: 4819767

IwataReuyl03: Iwata-Reuyl D, Math SK, Desai SB, Poulter CD (2003). "Bacterial phytoene synthase: molecular cloning, expression, and characterization of Erwinia herbicola phytoene synthase." Biochemistry 42(11);3359-65. PMID: 12641468

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Misawa94: Misawa N, Truesdale MR, Sandmann G, Fraser PD, Bird C, Schuch W, Bramley PM (1994). "Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, phytoene formation in vivo and in vitro, and functional analysis of the various truncated gene products." J Biochem 116(5);980-5. PMID: 7896759

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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