|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions → Composite Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 126.96.36.1994
Enzymes and Genes:
sesquiterpene dehydrogenase ( Cichorium intybus )
germacrene A alcohol dehydrogenase/hydroxylase : GAO1 ( Barnadesia spinosa )
germacrene A alcohol dehydrogenase/hydroxylase : GAO1 ( Cichorium intybus )
germacrene A alcohol dehydrogenase/hydroxylase : GAO1 ( Lactuca sativa )
germacrene A oxidase : GAO ( Tanacetum parthenium )
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: germacrene A alcohol dehydrogenase
germacra-1(10),4,11(13)-trien-12-ol + NADP+ → germacra-1(10),4,11(13)-trien-12-al + NADPH + H+ ,
germacra-1(10),4,11(13)-trien-12-al + NADP+ + H2O → germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H+
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -14.610046 [Latendresse13]
Enzyme Commission Summary:
This is an overall reaction that includes the two reactions listed above.
Unification Links: Rhea:30670
deKraker01: de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ (2001). "Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis." Plant Physiol 125(4);1930-40. PMID: 11299372
Nguyen10a: Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae." J Biol Chem 285(22);16588-98. PMID: 20351109
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