MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions Composite Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Barnadesia spinosa : germacrene A alcohol dehydrogenase/hydroxylase Inferred from experiment : GAO1
Cichorium intybus : germacrene A alcohol dehydrogenase/hydroxylase Inferred from experiment : GAO1
sesquiterpene dehydrogenase
Lactuca sativa : germacrene A alcohol dehydrogenase/hydroxylase Inferred from experiment : GAO1
Tanacetum parthenium : germacrene A oxidase : GAO

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: germacrene A alcohol dehydrogenase

Taxonomic Range: Viridiplantae

germacra-1(10),4,11(13)-trien-12-ol + NADP+ → germacra-1(10),4,11(13)-trien-12-al + NADPH + H+ ,
germacra-1(10),4,11(13)-trien-12-al + NADP+ + H2O → germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H+

Standard Gibbs Free Energy (ΔrG in kcal/mol): -14.610046 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This is an overall reaction that includes the two reactions listed above.

In Lactuca sativa EC is a mutifunctional enzyme that also catalyzes EC, germacrene A hydroxylase [Nguyen10a].

Citations: [deKraker01]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: Rhea:30670

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Created 15-Jun-2011 by Caspi R , SRI International


deKraker01: de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ (2001). "Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis." Plant Physiol 125(4);1930-40. PMID: 11299372

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Nguyen10a: Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae." J Biol Chem 285(22);16588-98. PMID: 20351109

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri Jul 31, 2015, BIOCYC13B.