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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Reaction: 2.1.1.224

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions

EC Number: 2.1.1.224

Enzymes and Genes:
23S rRNA (adenine2503-C8)-methyltransferase Inferred from experiment : cfr ( Staphylococcus sciuri )

In Pathway: linezolid resistance

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 23S rRNA (adenine2503-C8)-methyltransferase

Enzyme Commission Synonyms: Cfr (gene name)

Standard Gibbs Free Energy (ΔrG in kcal/mol): -50.38626 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Contains an [4Fe-4S] cluster [Giessing09]. Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics [Giessing09]. The enzyme methylates adenosine by a radical mechanism with CH2 from S-adenosyl-L-methionine and retention of the hydrogen at C-8 of adenine2503 of 23S rRNA. It will also methylate 2-methyladenine2503 of 23S rRNA. cf. EC 2.1.1.192 (23S rRNA (adenine2503-C2)-methyltransferase). This enzyme is a member of the 'AdoMet radical ' (radical SAM) family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group.

Citations: [Kaminska10, Yan10, Yan11a, Grove11, Boal11]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:2.1.1.224 , ENZYME:EC:2.1.1.224 , IUBMB-ExplorEnz:EC:2.1.1.224

Credits:
Created 20-Jun-2011 by Kothari A , SRI International


References

Boal11: Boal AK, Grove TL, McLaughlin MI, Yennawar NH, Booker SJ, Rosenzweig AC (2011). "Structural basis for methyl transfer by a radical SAM enzyme." Science 332(6033);1089-92. PMID: 21527678

Giessing09: Giessing AM, Jensen SS, Rasmussen A, Hansen LH, Gondela A, Long K, Vester B, Kirpekar F (2009). "Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria." RNA 15(2);327-36. PMID: 19144912

Grove11: Grove TL, Benner JS, Radle MI, Ahlum JH, Landgraf BJ, Krebs C, Booker SJ (2011). "A radically different mechanism for S-adenosylmethionine-dependent methyltransferases." Science 332(6029);604-7. PMID: 21415317

Kaminska10: Kaminska KH, Purta E, Hansen LH, Bujnicki JM, Vester B, Long KS (2010). "Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria." Nucleic Acids Res 38(5);1652-63. PMID: 20007606

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Yan10: Yan F, LaMarre JM, Rohrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA." J Am Chem Soc 132(11);3953-64. PMID: 20184321

Yan11a: Yan F, Fujimori DG (2011). "RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift." Proc Natl Acad Sci U S A 108(10);3930-4. PMID: 21368151


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc14.