|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
Enzymes and Genes:
|Mycobacterium tuberculosis :||3-oxocholest-4-en-26-oate--CoA ligase
|Rhodococcus rhodochrous :||3-oxocholest-4-en-26-oate–CoA ligase
|Saccharomyces cerevisiae :||medium-chain acyl-CoA synthetase
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Mass balance status: Balanced.
Enzyme Commission Primary Name: 3-oxocholest-4-en-26-oate—CoA ligase
Enzyme Commission Synonyms: fadD19 (gene name)
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): -12.71167 [Latendresse13]
Enzyme Commission Summary:
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3β-hydroxycholest-5-en-26-oate can also be used as substrate.
Wilbrink11: Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R (2011). "FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains." Appl Environ Microbiol 77(13);4455-64. PMID: 21602385
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