MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Mycobacterium tuberculosis : 3-oxocholest-4-en-26-oate--CoA ligase Inferred from experiment : fadD19
Rhodococcus rhodochrous : 3-oxocholest-4-en-26-oate–CoA ligase Inferred from experiment : fadD19
Saccharomyces cerevisiae : medium-chain acyl-CoA synthetase : FAA2

In Pathway: cholesterol degradation to androstenedione II (cholesterol dehydrogenase) , cholesterol degradation to androstenedione I (cholesterol oxidase)

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 3-oxocholest-4-en-26-oate—CoA ligase

Enzyme Commission Synonyms: fadD19 (gene name)

Standard Gibbs Free Energy (ΔrG in kcal/mol): -12.71167 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3β-hydroxycholest-5-en-26-oate can also be used as substrate.

Citations: [Casabon14, Wilbrink11]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Created 04-Oct-2011 by Caspi R , SRI International


Casabon14: Casabon I, Swain K, Crowe AM, Eltis LD, Mohn WW (2014). "Actinobacterial acyl coenzyme a synthetases involved in steroid side-chain catabolism." J Bacteriol 196(3);579-87. PMID: 24244004

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Wilbrink11: Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R (2011). "FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains." Appl Environ Microbiol 77(13);4455-64. PMID: 21602385

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.