Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 4.2.1.83

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 4.2.1.83

Enzymes and Genes:
4-oxalmesaconate hydratase Inferred from experiment : galB ( Pseudomonas putida KT2440 )
4-carboxy-2-hydroxymuconate hydratase Inferred by computational analysis : pmdE ( Comamonas testosteroni )
4-oxalomesaconate hydratase Inferred from experiment : ligJ ( Sphingomonas sp. SYK6 )
4-oxalomesaconate hydratase Inferred from experiment : proH ( Pseudomonas straminea )

In Pathway: protocatechuate degradation I (meta-cleavage pathway) , methylgallate degradation , gallate degradation I , gallate degradation II

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 4-oxalomesaconate hydratase

Enzyme Commission Synonyms: 4-oxalmesaconate hydratase, 4-carboxy-2-oxohexenedioate hydratase, 4-carboxy-2-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase, oxalmesaconate hydratase, γ-oxalmesaconate hydratase, 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase, LigJ, GalB

Standard Gibbs Free Energy (ΔrG in kcal/mol): 6.423004 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This enzyme participates in the degradation of protocatechuate (via the meta-cleavage pathway), syringate and gallate [Maruyama83, Maruyama85, Hara00]. It accepts the enol-form of 4-oxalomesaconate, 2-hydroxy-4-carboxy-hexa-2,4-dienedioate [Nogales11].

Gene-Reaction Schematic: ?

Unification Links: KEGG:R04478 , Rhea:17401

Relationship Links: BRENDA:EC:4.2.1.83 , ENZYME:EC:4.2.1.83 , IUBMB-ExplorEnz:EC:4.2.1.83

Credits:
Revised 24-Nov-2010 by Caspi R , SRI International


References

Hara00: Hara H, Masai E, Katayama Y, Fukuda M (2000). "The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6." J Bacteriol 182(24);6950-7. PMID: 11092855

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Maruyama83: Maruyama K (1983). "Enzymes responsible for degradation of 4-oxalmesaconic acid in Pseudomonas ochraceae." J Biochem (Tokyo) 93(2);567-74. PMID: 6841354

Maruyama85: Maruyama K (1985). "Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate." Biochem Biophys Res Commun 128(1);271-7. PMID: 3985968

Nogales11: Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E (2011). "Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida." Mol Microbiol 79(2);359-74. PMID: 21219457


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.