MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Comamonas testosteroni : 4-carboxy-2-hydroxymuconate hydratase Inferred by computational analysis : pmdE
Pseudomonas putida KT2440 : 4-oxalmesaconate hydratase Inferred from experiment : galB
Pseudomonas straminea : 4-oxalomesaconate hydratase Inferred from experiment : proH
Sphingomonas sp. SYK6 : 4-oxalomesaconate hydratase Inferred from experiment : ligJ

In Pathway: protocatechuate degradation I (meta-cleavage pathway) , methylgallate degradation , gallate degradation I , gallate degradation II

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 4-oxalomesaconate hydratase

Enzyme Commission Synonyms: 4-oxalmesaconate hydratase, 4-carboxy-2-oxohexenedioate hydratase, 4-carboxy-2-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase, oxalmesaconate hydratase, γ-oxalmesaconate hydratase, 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase, LigJ, GalB

Standard Gibbs Free Energy (ΔrG in kcal/mol): 6.423004 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This enzyme participates in the degradation of protocatechuate (via the meta-cleavage pathway), syringate and gallate [Maruyama83, Maruyama85, Hara00]. It accepts the enol-form of 4-oxalomesaconate, 2-hydroxy-4-carboxy-hexa-2,4-dienedioate [Nogales11].

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: KEGG:R04478 , Rhea:17401

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Revised 24-Nov-2010 by Caspi R , SRI International


Hara00: Hara H, Masai E, Katayama Y, Fukuda M (2000). "The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6." J Bacteriol 182(24);6950-7. PMID: 11092855

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Maruyama83: Maruyama K (1983). "Enzymes responsible for degradation of 4-oxalmesaconic acid in Pseudomonas ochraceae." J Biochem (Tokyo) 93(2);567-74. PMID: 6841354

Maruyama85: Maruyama K (1985). "Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate." Biochem Biophys Res Commun 128(1);271-7. PMID: 3985968

Nogales11: Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E (2011). "Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida." Mol Microbiol 79(2);359-74. PMID: 21219457

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.