MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Comamonas testosteroni: 4-carboxy-2-hydroxymuconate hydrataseInferred by computational analysis: pmdE
Pseudomonas putida KT2440: 4-oxalmesaconate hydrataseInferred from experiment: galB
Pseudomonas straminea: 4-oxalomesaconate hydrataseInferred from experiment: proH
Sphingomonas sp. SYK6: 4-oxalomesaconate hydrataseInferred from experiment: ligJ

In Pathway: protocatechuate degradation I (meta-cleavage pathway), methylgallate degradation, gallate degradation I, gallate degradation II

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 4-oxalomesaconate hydratase

Enzyme Commission Synonyms: 4-oxalmesaconate hydratase, 4-carboxy-2-oxohexenedioate hydratase, 4-carboxy-2-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase, oxalmesaconate hydratase, γ-oxalmesaconate hydratase, 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase, LigJ, GalB

Standard Gibbs Free Energy (ΔrG): 6.423004 kcal/molInferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
This enzyme participates in the degradation of protocatechuate (via the meta-cleavage pathway), syringate and gallate [Maruyama83, Maruyama85, Hara00]. It accepts the enol-form of 4-oxalomesaconate, 2-hydroxy-4-carboxy-hexa-2,4-dienedioate [Nogales11].

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R04478, Rhea:17401

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Revised 24-Nov-2010 by Caspi R, SRI International


Hara00: Hara H, Masai E, Katayama Y, Fukuda M (2000). "The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6." J Bacteriol 182(24);6950-7. PMID: 11092855

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Maruyama83: Maruyama K (1983). "Enzymes responsible for degradation of 4-oxalmesaconic acid in Pseudomonas ochraceae." J Biochem (Tokyo) 93(2);567-74. PMID: 6841354

Maruyama85: Maruyama K (1985). "Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate." Biochem Biophys Res Commun 128(1);271-7. PMID: 3985968

Nogales11: Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E (2011). "Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida." Mol Microbiol 79(2);359-74. PMID: 21219457

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 20.0 (software by SRI International) on Fri May 6, 2016, BIOCYC14.