MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Saccharomyces cerevisiae: dolichyl-P-Man:Man5GlcNAc2-PP-dolichol α-1,3-mannosyltransferaseInferred from experiment: ALG3

In Pathway: protein N-glycosylation (eukaryotic, high mannose)

Supersedes EC number:

Reaction Locations: rough endoplasmic reticulum lumen

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: dolichyl-P-Man:Man5GlcNAc2-PP-dolichol α-1,3-mannosyltransferase

Enzyme Commission Synonyms: Man5GlcNAc2-PP-Dol mannosyltransferase, ALG3, dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase, Not56-like protein, Alg3 α-1,3-mannosyl transferase, Dol-P-Man:Man5GlcNAc2-PP-Dol α-1,3-mannosyltransferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.52124Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from a (mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolichol to a (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol on the lumenal side use dolichyl β-D-mannosyl phosphate. The first step of this assembly pathway on the luminal side of the endoplasmic reticulum is catalysed by ALG3.

Citations: [Sharma01, Cipollo00]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R06258, Rhea:29530

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Revised 06-Dec-2010 by Caspi R, SRI International


Cipollo00: Cipollo JF, Trimble RB (2000). "The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing." J Biol Chem 275(6);4267-77. PMID: 10660594

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Sharma01: Sharma CB, Knauer R, Lehle L (2001). "Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase." Biol Chem 382(2);321-8. PMID: 11308030

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Wed Nov 25, 2015, biocyc12.