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Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 2.4.1.258

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 2.4.1.258

Enzymes and Genes:
Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase Inferred from experiment : ALG3 ( Saccharomyces cerevisiae )

In Pathway: dolichyl-diphosphooligosaccharide biosynthesis

Supersedes EC number: 2.4.1.130

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: dolichyl-P-Man:Man5GlcNAc2-PP-dolichol α-1,3-mannosyltransferase

Enzyme Commission Synonyms: Man5GlcNAc2-PP-Dol mannosyltransferase, ALG3, dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase, Not56-like protein, Alg3 α-1,3-mannosyl transferase, Dol-P-Man:Man5GlcNAc2-PP-Dol α-1,3-mannosyltransferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.52124 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from a (mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolichol to a (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol on the lumenal side use dolichyl β-D-mannosyl phosphate. The first step of this assembly pathway on the luminal side of the endoplasmic reticulum is catalysed by ALG3.

Citations: [Sharma01, Cipollo00]

Gene-Reaction Schematic: ?

Unification Links: Rhea:29530

Relationship Links: BRENDA:EC:2.4.1.258 , ENZYME:EC:2.4.1.258 , IUBMB-ExplorEnz:EC:2.4.1.258

Credits:
Revised 06-Dec-2010 by Caspi R , SRI International


References

Cipollo00: Cipollo JF, Trimble RB (2000). "The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing." J Biol Chem 275(6);4267-77. PMID: 10660594

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Sharma01: Sharma CB, Knauer R, Lehle L (2001). "Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase." Biol Chem 382(2);321-8. PMID: 11308030


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC14B.