MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Saccharomyces cerevisiae: (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol glucosyltransferaseInferred from experiment: ALG6

In Pathway: protein N-glycosylation (eukaryotic, high mannose)

Reaction Locations: rough endoplasmic reticulum lumen

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol α-1,3-glucosyltransferase

Enzyme Commission Synonyms: ALG6, Dol-P-Glc:Man9GlcNAc2-PP-Dol α-1,3-glucosyltransferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.5214844Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from a (mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolichol to a (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol on the lumenal side use dolichyl β-D-mannosyl phosphate. Subsequent addition of the three glucose residues by EC, EC and EC represents the final stage of the lipid-linked oligosaccharide assembly.

Citations: [Reiss96, Runge84 , Westphal03]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R06262, Rhea:30638

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Revised 06-Dec-2010 by Caspi R, SRI International


Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Reiss96: Reiss G, te Heesen S, Zimmerman J, Robbins PW, Aebi M (1996). "Isolation of the ALG6 locus of Saccharomyces cerevisiae required for glucosylation in the N-linked glycosylation pathway." Glycobiology 6(5);493-8. PMID: 8877369

Runge84: Runge KW, Huffaker TC, Robbins PW (1984). "Two yeast mutations in glucosylation steps of the asparagine glycosylation pathway." J Biol Chem 259(1);412-7. PMID: 6423630

Westphal03: Westphal V, Xiao M, Kwok PY, Freeze HH (2003). "Identification of a frequent variant in ALG6, the cause of Congenital Disorder of Glycosylation-Ic." Hum Mutat 22(5);420-1. PMID: 14517965

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Tue Nov 24, 2015, BIOCYC13A.