MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Saccharomyces cerevisiae : (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol glucosyltransferase Inferred from experiment : ALG6

In Pathway: protein N-glycosylation (eukaryotic, high mannose)

Reaction Locations: rough endoplasmic reticulum lumen

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol α-1,3-glucosyltransferase

Enzyme Commission Synonyms: ALG6, Dol-P-Glc:Man9GlcNAc2-PP-Dol α-1,3-glucosyltransferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.5214844 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from a (mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolichol to a (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol on the lumenal side use dolichyl β-D-mannosyl phosphate. Subsequent addition of the three glucose residues by EC, EC and EC represents the final stage of the lipid-linked oligosaccharide assembly.

Citations: [Reiss96, Runge84, Westphal03]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Unification Links: Rhea:30638

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Revised 06-Dec-2010 by Caspi R , SRI International


Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Reiss96: Reiss G, te Heesen S, Zimmerman J, Robbins PW, Aebi M (1996). "Isolation of the ALG6 locus of Saccharomyces cerevisiae required for glucosylation in the N-linked glycosylation pathway." Glycobiology 6(5);493-8. PMID: 8877369

Runge84: Runge KW, Huffaker TC, Robbins PW (1984). "Two yeast mutations in glucosylation steps of the asparagine glycosylation pathway." J Biol Chem 259(1);412-7. PMID: 6423630

Westphal03: Westphal V, Xiao M, Kwok PY, Freeze HH (2003). "Identification of a frequent variant in ALG6, the cause of Congenital Disorder of Glycosylation-Ic." Hum Mutat 22(5);420-1. PMID: 14517965

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Jul 30, 2015, biocyc14.