|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 184.108.40.2067
Enzymes and Genes:
|Saccharomyces cerevisiae:||(mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol glucosyltransferase: ALG6|
Reaction Locations: rough endoplasmic reticulum lumen
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol α-1,3-glucosyltransferase
Enzyme Commission Synonyms: ALG6, Dol-P-Glc:Man9GlcNAc2-PP-Dol α-1,3-glucosyltransferase
Standard Gibbs Free Energy (ΔrG'° in kcal/mol): 7.5214844 [Latendresse13]
Enzyme Commission Summary:
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from a (mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolichol to a (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol on the lumenal side use dolichyl β-D-mannosyl phosphate. Subsequent addition of the three glucose residues by EC 220.127.116.117, EC 18.104.22.1685 and EC 22.214.171.1246 represents the final stage of the lipid-linked oligosaccharide assembly.
Reiss96: Reiss G, te Heesen S, Zimmerman J, Robbins PW, Aebi M (1996). "Isolation of the ALG6 locus of Saccharomyces cerevisiae required for glucosylation in the N-linked glycosylation pathway." Glycobiology 6(5);493-8. PMID: 8877369
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