MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655: purine nucleotidaseInferred from experiment: yrfG
UMP phosphatase: umpH
broad specificity 5'(3')-nucleotidase and polyphosphatase: umpG
acid phosphatase / phosphotransferase: aphA
Homo sapiens: cytosolic 5'-nucleotidase IIInferred from experiment: NT5C2
5'-nucleotidase: NT5E
Solanum tuberosum: 5'-nucleotidaseInferred from experiment

In Pathway: guanosine nucleotides degradation II, guanosine nucleotides degradation I, guanosine nucleotides degradation III

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 5′-nucleotidase

Enzyme Commission Synonyms: uridine 5'-nucleotidase, 5'-adenylic phosphatase, adenosine 5'-phosphatase, AMP phosphatase, adenosine monophosphatase, 5'-mononucleotidase, AMPase, UMPase, snake venom 5'-nucleotidase, thimidine monophosphate nucleotidase, 5'-AMPase, 5'-AMP nucleotidase, AMP phosphohydrolase, IMP 5'-nucleotidase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.2170105Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Wide specificity for 5-nucleotides.

Citations: [Gulland38, Heppel51, Segal60]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reaction of [a ribonucleoside 5'-monophosphate + H2O → a ribonucleoside + phosphate] (
i1: GMP + H2O → guanosine + phosphate (

Unification Links: KEGG:R01227, Rhea:27714

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Gulland38: Gulland JM, Jackson EM (1938). "5-Nucleotidase." Biochem J 32(3);597-601. PMID: 16746659

Heppel51: Heppel LA, Hilmore RJ (1951). "Purification and properties of 5-nucleotidase." J Biol Chem 188(2);665-76. PMID: 14824154

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Segal60: Segal HL, Brenner BM (1960). "5'-Nucleotidase of rat liver microsomes." J Biol Chem 235;471-4. PMID: 14444527

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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