MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Homo sapiens: hormone-sensitive lipaseInferred from experiment: LIPE
Saccharomyces cerevisiae: steryl ester hydrolase: TGL1

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Instance reactions:
ergosteryl oleate + H2O → ergosterol + oleate + H+ (

lanosteryl oleate + H2O → lanosterol + oleate + H+ (

Enzyme Commission Primary Name: sterol esterase

Enzyme Commission Synonyms: cholesterol esterase, cholesteryl ester synthase, triterpenol esterase, cholesteryl esterase, cholesteryl ester hydrolase, sterol ester hydrolase, cholesterol ester hydrolase, cholesterase, acylcholesterol lipase

Taxonomic Range: Fungi, Metazoa

Standard Gibbs Free Energy (ΔrG in kcal/mol): -13.038246Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
A group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts.

Citations: [Hyun69, Okawa77, Vahouny68]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reaction of [an acyl-CoA + a 1-acyl 2-lyso-phosphatidylcholine ↔ a phosphatidylcholine + coenzyme A] (
i3: palmitoyl-CoA + 1-16:0-2-lysophosphatidylcholine ↔ 1,2-dipalmitoyl-phosphatidylcholine + coenzyme A (

Instance reaction of [a phosphatidylcholine + H2O → a 1-acyl 2-lyso-phosphatidylcholine + a long-chain fatty acid + H+] (
i12: 1,2-dipalmitoyl-phosphatidylcholine + H2O → 1-16:0-2-lysophosphatidylcholine + palmitate + H+ (

Instance reactions of [a long-chain acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate → a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A] (
i5: palmitoleoyl-CoA + 1-myristoylglycerol 3-phosphate → 1-myristoyl-2-palmitoleoyl phosphatidate + coenzyme A (

i6: palmitoleoyl-CoA + 1-cis-vaccenoylglycerol-3-phosphate → 1-cis-vaccenoyl-2-palmitoleoyl phosphatidate + coenzyme A (

i7: palmitoleoyl-CoA + 1-palmitoylglycerol 3-phosphate → 1-palmitoyl-2-palmitoleoyl phosphatidate + coenzyme A (

i8: 1-palmitoylglycerol 3-phosphate + myristoyl-CoA → 1-palmitoyl-2-myristoyl phosphatidate + coenzyme A (

i9: myristoyl-CoA + 1-myristoylglycerol 3-phosphate → dimyristoyl phosphatidate + coenzyme A (

i10: cis-vaccenoyl-CoA + 1-cis-vaccenoylglycerol-3-phosphate → 1,2-dicis-vaccenoyl-phosphatidate + coenzyme A (

i11: cis-vaccenoyl-CoA + 1-palmitoylglycerol 3-phosphate → 1-palmitoyl-2-cis-vaccenoyl phosphatidate + coenzyme A (

Instance reactions of [a steryl-ester + H2O → a fatty acid + a sterol + H+] (
i13: ergosteryl oleate + H2O → ergosterol + oleate + H+ (

i14: lanosteryl oleate + H2O → lanosterol + oleate + H+ (

Instance reaction of [a triglyceride + H2O → a 1,2-diglyceride + a fatty acid + H+] (
i4: tributyrin + H2O → 1,2-dibutyrin + butanoate + H+ (

Unification Links: KEGG:R02115

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:P07882, UniProt:RELATED-TO:P30122, UniProt:RELATED-TO:P38571, UniProt:RELATED-TO:Q64285, UniProt:RELATED-TO:Q64571


Hyun69: Hyun J, Kothari H, Herm E, Mortenson J, Treadwell CR, Vahouny GV (1969). "Purification and properties of pancreatic juice cholesterol esterase." J Biol Chem 244(7);1937-45. PMID: 5780846

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Okawa77: Okawa Y, Yamaguchi T (1977). "Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties." J Biochem 81(5);1209-15. PMID: 19426

Vahouny68: Vahouny GV, Treadwell CR (1968). "Enzymatic synthesis and hydrolysis of cholesterol esters." Methods Biochem Anal 16;219-72. PMID: 4877146

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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