Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Reaction: 3.1.1.13

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.1.1.13

Enzymes and Genes:
steryl ester hydrolase : TGL1 ( Saccharomyces cerevisiae )
hormone-sensitive lipase Inferred from experiment : LIPE ( Homo sapiens )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Instance reaction:
lanosteryl oleate + H2O → lanosterol + oleate + H+ (3.1.1.13)

Enzyme Commission Primary Name: sterol esterase

Enzyme Commission Synonyms: cholesterol esterase, cholesteryl ester synthase, triterpenol esterase, cholesteryl esterase, cholesteryl ester hydrolase, sterol ester hydrolase, cholesterol ester hydrolase, cholesterase, acylcholesterol lipase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -13.038246 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
A group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts.

Citations: [Hyun69, Okawa77, Vahouny68]

Gene-Reaction Schematic: ?

Instance reaction of [an L-1-phosphatidyl-ethanolamine[periplasmic space] + H2O[periplasmic space] → a fatty acid[periplasmic space] + a 2-lyso-phosphatidyl-ethanolamine[periplasmic space] + H+[periplasmic space]] (3.1.1.4):
i3: 1-18:1-2-18:1-phosphatidylethanolamine + H2O → 1-18:1-lysophosphatidylethanolamine + oleate + H+ (3.1.1.4)

Instance reaction of [a phosphatidylcholine + H2O → a 2-lyso-phosphatidylcholine + a fatty acid + H+] (3.1.1.4):
i6: 1,2-dipalmitoyl-phosphatidylcholine + H2O → 1-16:0-2-lysophosphatidylcholine + palmitate + H+ (3.1.1.4)

Instance reaction of [an acyl-CoA + a 2-lyso-phosphatidylcholine ↔ a phosphatidylcholine + coenzyme A] (2.3.1.23):
i4: 1-16:0-2-lysophosphatidylcholine + palmitoyl-CoA ↔ 1,2-dipalmitoyl-phosphatidylcholine + coenzyme A (2.3.1.23)

Instance reaction of [a triglyceride + H2O → a 1,2-diglyceride + a fatty acid + H+] (3.1.1.3):
i5: tributyrin + H2O → 1,2-dibutyrin + butanoate + H+ (3.1.1.3)

Instance reaction of [a steryl-ester + H2O → a fatty acid + a sterol + H+] (3.1.1.13):
i7: lanosteryl oleate + H2O → lanosterol + oleate + H+ (3.1.1.13)

Unification Links: KEGG:R02115

Relationship Links: BRENDA:EC:3.1.1.13 , ENZYME:EC:3.1.1.13 , IUBMB-ExplorEnz:EC:3.1.1.13 , UniProt:RELATED-TO:P07882 , UniProt:RELATED-TO:P30122 , UniProt:RELATED-TO:P38571 , UniProt:RELATED-TO:Q64285 , UniProt:RELATED-TO:Q64571


References

Hyun69: Hyun J, Kothari H, Herm E, Mortenson J, Treadwell CR, Vahouny GV (1969). "Purification and properties of pancreatic juice cholesterol esterase." J Biol Chem 244(7);1937-45. PMID: 5780846

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Okawa77: Okawa Y, Yamaguchi T (1977). "Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties." J Biochem 81(5);1209-15. PMID: 19426

Vahouny68: Vahouny GV, Treadwell CR (1968). "Enzymatic synthesis and hydrolysis of cholesterol esters." Methods Biochem Anal 16;219-72. PMID: 4877146


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc14.