MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:

Homo sapiens : hormone-sensitive lipase Inferred from experiment : LIPE
Saccharomyces cerevisiae : steryl ester hydrolase : TGL1

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Instance reactions:
ergosteryl oleate + H2O → ergosterol + oleate + H+ (

lanosteryl oleate + H2O → lanosterol + oleate + H+ (

Enzyme Commission Primary Name: sterol esterase

Enzyme Commission Synonyms: cholesterol esterase, cholesteryl ester synthase, triterpenol esterase, cholesteryl esterase, cholesteryl ester hydrolase, sterol ester hydrolase, cholesterol ester hydrolase, cholesterase, acylcholesterol lipase

Taxonomic Range: Fungi , Metazoa

Standard Gibbs Free Energy (ΔrG in kcal/mol): -13.038246 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
A group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts.

Citations: [Hyun69, Okawa77, Vahouny68]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reaction of [a phosphatidylcholine + H2O → a 1-acyl 2-lyso-phosphatidylcholine + a long-chain fatty acid + H+] (
i5: 1,2-dipalmitoyl-phosphatidylcholine + H2O → 1-16:0-2-lysophosphatidylcholine + palmitate + H+ (

Instance reaction of [an acyl-CoA + a 1-acyl 2-lyso-phosphatidylcholine ↔ a phosphatidylcholine + coenzyme A] (
i3: palmitoyl-CoA + 1-16:0-2-lysophosphatidylcholine ↔ 1,2-dipalmitoyl-phosphatidylcholine + coenzyme A (

Instance reaction of [a triglyceride + H2O → a 1,2-diglyceride + a fatty acid + H+] (
i4: tributyrin + H2O → 1,2-dibutyrin + butanoate + H+ (

Instance reaction of [an L-1-phosphatidylethanolamine[periplasmic space] + H2O[periplasmic space] → a fatty acid[periplasmic space] + a 1-acyl 2-lyso-phosphatidylethanolamine[periplasmic space] + H+[periplasmic space]] (
i2: 1-18:1-2-18:1-phosphatidylethanolamine + H2O → 1-18:1-lysophosphatidylethanolamine + oleate + H+ (

Instance reactions of [a steryl-ester + H2O → a fatty acid + a sterol + H+] (
i7: ergosteryl oleate + H2O → ergosterol + oleate + H+ (

i8: lanosteryl oleate + H2O → lanosterol + oleate + H+ (

Unification Links: KEGG:R02115

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:P07882 , UniProt:RELATED-TO:P30122 , UniProt:RELATED-TO:P38571 , UniProt:RELATED-TO:Q64285 , UniProt:RELATED-TO:Q64571


Hyun69: Hyun J, Kothari H, Herm E, Mortenson J, Treadwell CR, Vahouny GV (1969). "Purification and properties of pancreatic juice cholesterol esterase." J Biol Chem 244(7);1937-45. PMID: 5780846

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Okawa77: Okawa Y, Yamaguchi T (1977). "Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties." J Biochem 81(5);1209-15. PMID: 19426

Vahouny68: Vahouny GV, Treadwell CR (1968). "Enzymatic synthesis and hydrolysis of cholesterol esters." Methods Biochem Anal 16;219-72. PMID: 4877146

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc12.