Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Reaction: 3.1.1.13

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 3.1.1.13

Enzymes and Genes:
steryl ester hydrolase : TGL1 ( Saccharomyces cerevisiae )
hormone-sensitive lipase Inferred from experiment : LIPE ( Homo sapiens )

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Instance reaction:
lanosteryl oleate + H2O → lanosterol + oleate + H+ (3.1.1.13)

Enzyme Commission Primary Name: sterol esterase

Enzyme Commission Synonyms: cholesterol esterase, cholesteryl ester synthase, triterpenol esterase, cholesteryl esterase, cholesteryl ester hydrolase, sterol ester hydrolase, cholesterol ester hydrolase, cholesterase, acylcholesterol lipase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -13.038246 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
A group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts.

Citations: [Hyun69, Okawa77, Vahouny68]

Gene-Reaction Schematic: ?

Instance reaction of [an acyl-CoA + a 2-lyso-phosphatidylcholine ↔ a phosphatidylcholine + coenzyme A] (2.3.1.23):
i4: 1-16:0-2-lysophosphatidylcholine + palmitoyl-CoA ↔ 1,2-dipalmitoyl-phosphatidylcholine + coenzyme A (2.3.1.23)

Instance reaction of [a phosphatidylcholine + H2O → a 2-lyso-phosphatidylcholine + a fatty acid + H+] (3.1.1.4):
i6: 1,2-dipalmitoyl-phosphatidylcholine + H2O → 1-16:0-2-lysophosphatidylcholine + palmitate + H+ (3.1.1.4)

Instance reaction of [an L-1-phosphatidyl-ethanolamine[periplasmic space] + H2O[periplasmic space] → a fatty acid[periplasmic space] + a 2-lyso-phosphatidyl-ethanolamine[periplasmic space] + H+[periplasmic space]] (3.1.1.4):
i3: 1-18:1-2-18:1-phosphatidylethanolamine + H2O → 1-18:1-lysophosphatidylethanolamine + oleate + H+ (3.1.1.4)

Instance reaction of [a triglyceride + H2O → a 1,2-diglyceride + a fatty acid + H+] (3.1.1.3):
i5: tributyrin + H2O → 1,2-dibutyrin + butanoate + H+ (3.1.1.3)

Instance reaction of [a steryl-ester + H2O → a fatty acid + a sterol + H+] (3.1.1.13):
i7: lanosteryl oleate + H2O → lanosterol + oleate + H+ (3.1.1.13)

Unification Links: KEGG:R02115

Relationship Links: BRENDA:EC:3.1.1.13 , ENZYME:EC:3.1.1.13 , IUBMB-ExplorEnz:EC:3.1.1.13 , UniProt:RELATED-TO:P07882 , UniProt:RELATED-TO:P30122 , UniProt:RELATED-TO:P38571 , UniProt:RELATED-TO:Q64285 , UniProt:RELATED-TO:Q64571


References

Hyun69: Hyun J, Kothari H, Herm E, Mortenson J, Treadwell CR, Vahouny GV (1969). "Purification and properties of pancreatic juice cholesterol esterase." J Biol Chem 244(7);1937-45. PMID: 5780846

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Okawa77: Okawa Y, Yamaguchi T (1977). "Studies on sterol-ester hydrolase from Fusarium oxysporum. I. Partial purification and properties." J Biochem 81(5);1209-15. PMID: 19426

Vahouny68: Vahouny GV, Treadwell CR (1968). "Enzymatic synthesis and hydrolysis of cholesterol esters." Methods Biochem Anal 16;219-72. PMID: 4877146


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13A.