MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Escherichia coli K-12 substr. MG1655: 5'-ribonucleotidase: ushA
pyrimidine nucleotidase: yjjG
UMP phosphatase: umpH
broad specificity 5'(3')-nucleotidase and polyphosphatase: umpG
acid phosphatase / phosphotransferase: aphA
Homo sapiens: 5'-nucleotidaseInferred from experiment: NT5E
cytosolic 5'-nucleotidase IIInferred from experiment: NT5C2

In Pathway: adenosine nucleotides degradation I, urate biosynthesis/inosine 5'-phosphate degradation

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 5′-nucleotidase

Enzyme Commission Synonyms: uridine 5'-nucleotidase, 5'-adenylic phosphatase, adenosine 5'-phosphatase, AMP phosphatase, adenosine monophosphatase, 5'-mononucleotidase, AMPase, UMPase, snake venom 5'-nucleotidase, thimidine monophosphate nucleotidase, 5'-AMPase, 5'-AMP nucleotidase, AMP phosphohydrolase, IMP 5'-nucleotidase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 7.217041Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Wide specificity for 5-nucleotides.

Citations: [Gulland38, Heppel51, Segal60]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reaction of [a ribonucleoside 5'-monophosphate + H2O → a ribonucleoside + phosphate] (
i1: XMP + H2O → xanthosine + phosphate (

Unification Links: KEGG:R02719, Rhea:28530

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Gulland38: Gulland JM, Jackson EM (1938). "5-Nucleotidase." Biochem J 32(3);597-601. PMID: 16746659

Heppel51: Heppel LA, Hilmore RJ (1951). "Purification and properties of 5-nucleotidase." J Biol Chem 188(2);665-76. PMID: 14824154

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Segal60: Segal HL, Brenner BM (1960). "5'-Nucleotidase of rat liver microsomes." J Biol Chem 235;471-4. PMID: 14444527

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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